Abramo Francesca, Lazzarini Giulia, Pirone Andrea, Lenzi Carla, Albertini Sonia, Della Valle M Frederica, Schievano Carlo, Vannozzi Iacopo, Miragliotta Vincenzo
Department of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, Pisa, I-56124, Italy.
Endocrinology Unit, Azienda Ospedaliero-Universitaria Pisana, Via Roma 67, Pisa, I-56100, Italy.
Vet Dermatol. 2017 Oct;28(5):456-e104. doi: 10.1111/vde.12456. Epub 2017 Jun 5.
Ultramicronized palmitoylethanolamide (PEA-um) has been reported to reduce pruritus and skin lesions in dogs with moderate atopic dermatitis and pruritus.
HYPOTHESIS/OBJECTIVES: A canine ex vivo skin model was used to investigate the ability of PEA-um to counteract changes induced by compound 48/80, a well-known secretagogue that causes mast cell degranulation.
Normal skin was obtained from three donor dogs subjected to surgery for reasons unrelated to the study.
Cultured skin biopsy samples in triplicate were treated with 10 and 100 μg/mL compound 48/80, without or with 30 μM PEA-um. Mast cell (MC) degranulation, histamine release into the culture medium, local microvascular dilatation, epidermal thickness, keratinocyte proliferation and epidermal differentiation markers were evaluated.
Exposure of the skin organ culture to PEA-um 24 h before and 72 h concomitantly to compound 48/80 resulted in a significant decrease of degranulating MCs. PEA-um also reduced the histamine content in the culture medium by half, although the effect did not reach statistical significance. PEA-um significantly counteracted vasodilation induced by 100 μg/mL compound 48/80. Finally, PEA-um alone did not induce changes in epidermal thickness, differentiation markers, keratinocyte proliferation, MC density and/or degranulation.
Collectively, these results support the protective action PEA-um on the skin of dogs undergoing allergic changes.
据报道，超微化棕榈酰乙醇胺（PEA-um）可减轻中度特应性皮炎和瘙痒犬的瘙痒及皮肤损伤。
假设/目的：使用犬离体皮肤模型研究PEA-um对抗由化合物48/80诱导的变化的能力，化合物48/80是一种众所周知的促分泌素，可导致肥大细胞脱颗粒。
从三只因与研究无关的原因接受手术的供体犬获取正常皮肤。
将培养的皮肤活检样本一式三份，分别用10和100μg/mL的化合物48/80处理，不加或加30μM PEA-um。评估肥大细胞（MC）脱颗粒、组胺释放到培养基中、局部微血管扩张、表皮厚度、角质形成细胞增殖和表皮分化标志物。
在皮肤器官培养物中，在化合物48/80处理前24小时和处理期间72小时给予PEA-um，导致脱颗粒的MC显著减少。PEA-um还使培养基中的组胺含量降低了一半，尽管该效果未达到统计学意义。PEA-um显著对抗了100μg/mL化合物48/80诱导的血管舒张。最后，单独使用PEA-um不会引起表皮厚度、分化标志物、角质形成细胞增殖、MC密度和/或脱颗粒的变化。
总体而言，这些结果支持PEA-um对发生过敏变化的犬皮肤的保护作用。
