Moisseyev Ruslan, Pozharskiy Alexandr, Taskuzhina Aisha, Khusnitdinova Marina, Svanbayev Ualikhan, Sapakhova Zagipa, Gritsenko Dilyara
Laboratory of Molecular Biology, Institute of Plant Biology and Biotechnology, Almaty 050040, Kazakhstan.
Department of Molecular Biology and Genetics, Al-Farabi Kazakh National University, Almaty 050040, Kazakhstan.
Curr Issues Mol Biol. 2024 Oct 12;46(10):11326-11335. doi: 10.3390/cimb46100674.
Sugar beet hybrids are essential in modern agriculture due to their superior yields, disease resistance, and adaptability. This study investigates the role of the gene in conferring resistance to beet necrotic yellow vein virus (BNYVV) in 14 sugar beet hybrids cultivated in Kazakhstan, including local and European varieties. The gene, encoding a CC-NB-LRR protein, is a known resistance factor against BNYVV. Using RT-qPCR, we assessed expression and detected BNYVV in bait plants inoculated with virus-infested soil. Our findings identified two highly resistant varieties: the Kazakh cultivar 'Abulhair' and the French line 22b5006. Additionally, the Kazakh cultivar 'Pamyati Abugalieva' and the French hybrid 'Bunker' exhibited increased resistance, suggesting involvement of other resistance loci. Notably, the Danish hybrid 'Alando', despite resistance to rhizomania, did not effectively resist BNYVV, highlighting possible evasion of its genetic factors by local virus strains. Our results emphasize the importance of in resistance breeding programs and advocate for further research on additional resistance genes and the genetic variability of BNYVV in Kazakhstan. This work pioneers the molecular evaluation of BNYVV resistance in sugar beet in Kazakhstan, contributing to sustainable disease management and improved sugar beet production.
由于其高产、抗病性和适应性，甜菜杂交种在现代农业中至关重要。本研究调查了该基因在哈萨克斯坦种植的14个甜菜杂交种（包括当地品种和欧洲品种）中赋予对甜菜坏死黄脉病毒（BNYVV）抗性的作用。该基因编码一种CC-NB-LRR蛋白，是已知的抗BNYVV的抗性因子。我们使用RT-qPCR评估了该基因的表达，并在接种了受病毒侵染土壤的诱饵植物中检测到了BNYVV。我们的研究结果鉴定出了两个高抗品种：哈萨克品种“Abulhair”和法国品系22b5006。此外，哈萨克品种“Pamyati Abugalieva”和法国杂交种“Bunker”表现出增强的抗性，表明其他抗性位点也参与其中。值得注意的是，丹麦杂交种“Alando”尽管对根瘤病有抗性，但对BNYVV没有有效抗性，这突出表明当地病毒株可能规避了其遗传因子。我们的结果强调了该基因在抗性育种计划中的重要性，并倡导对哈萨克斯坦其他抗性基因和BNYVV的遗传变异性进行进一步研究。这项工作开创了哈萨克斯坦甜菜对BNYVV抗性的分子评估，有助于可持续的病害管理和提高甜菜产量。
