Département des sciences biologiques de l'environnement, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, Bejaia, 06000, Algerie; Laboratoire Biotechnologie, Environnement et Santé, Université de Jijel, Jijel, 18000, Algerie.
Département des sciences biologiques de l'environnement, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, Bejaia, 06000, Algerie; Laboratoire Biotechnologie, Environnement et Santé, Université de Jijel, Jijel, 18000, Algerie.
Biomed Pharmacother. 2017 Dec;96:1310-1316. doi: 10.1016/j.biopha.2017.11.081. Epub 2017 Dec 6.
The purpose of this study was to evaluate the protective effect of Pistacia lentiscus oil (PLO), known for its antioxidant properties, on chlorpyrifos (CPF)-induced alterations in the thyroid, reproductive hormone levels, and oxidative damage in the ovaries and thyroid of adult Wistar rats. The animals were treated with orally administered PLO (2 mL/kg), CPF (6.75 mg/kg), and a combination of CPF and PLO for 30 days. Serum levels of luteinizing hormone (LH), follicle-stimulating hormone (FSH), progesterone (Pg), estradiol (E), triiodothyronine (T3), thyroxine (T4), and thyroid-stimulating hormone (TSH) were assessed using chemiluminescence assay. Malondialdehyde (MDA), protein carbonyl (PC), and reduced glutathione (GSH) levels were examined in the ovaries and thyroid glands. The oil principal volatile compounds detected by gas chromatography analysis were: myrcene, α-pinene and limonene (26.21, 22.66 and 10.33%, respectively). No significant differences were observed between serum concentrations of TSH and FSH in the examined experimental groups. However, serum concentrations of LH, E, Pg, T3, and T4 decreased significantly in CPF-treated rats in comparison with the controls. The body weight and relative weight of ovaries and thyroids in this group were also significantly reduced. The MDA and PC content increased significantly, while the GSH content was markedly depressed in the thyroid and ovaries of rats treated with CPF. Co-administration of PLO and CPF effectively ameliorated the adverse effects; the oxidative damage was reduced and the levels of thyroid and reproductive hormones restored to a normal range. In conclusion, it appears that PLO substantially alleviates the CPF-induced oxidative damage and hormonal alterations.
本研究旨在评估具有抗氧化特性的乳香黄连木油（PLO）对毒死蜱（CPF）诱导的成年 Wistar 大鼠甲状腺、生殖激素水平和卵巢及甲状腺氧化损伤的保护作用。动物通过口服给予 PLO（2 mL/kg）、CPF（6.75 mg/kg）和 CPF 与 PLO 的组合进行治疗，持续 30 天。使用化学发光法评估血清黄体生成素（LH）、卵泡刺激素（FSH）、孕酮（Pg）、雌二醇（E）、三碘甲状腺原氨酸（T3）、甲状腺素（T4）和促甲状腺激素（TSH）的水平。在卵巢和甲状腺中检测丙二醛（MDA）、蛋白质羰基（PC）和还原型谷胱甘肽（GSH）的水平。通过气相色谱分析检测到油的主要挥发性化合物为：月桂烯、α-蒎烯和柠檬烯（分别为 26.21%、22.66%和 10.33%）。在所检查的实验组中，TSH 和 FSH 的血清浓度没有观察到显著差异。然而，与对照组相比，CPF 处理的大鼠血清 LH、E、Pg、T3 和 T4 浓度显著降低。该组大鼠的体重和卵巢、甲状腺的相对重量也显著降低。CPF 处理大鼠的甲状腺和卵巢中的 MDA 和 PC 含量显著增加，而 GSH 含量明显降低。PLO 与 CPF 联合给药可有效改善不良反应；氧化损伤减轻，甲状腺和生殖激素水平恢复正常范围。综上所述，PLO 可显著减轻 CPF 诱导的氧化损伤和激素改变。
