Kochan Ewa, Szymczyk Piotr, Kuźma Łukasz, Lipert Anna, Szymańska Grażyna
Pharmaceutical Biotechnology Department, Medical University of Lodz, Muszyńskiego 1, Lodz 90-151, Poland.
Department of Biology and Pharmaceutical Botany, Medical University of Lodz, Muszyńskiego l, Lodz 90-151, Poland.
Molecules. 2017 May 26;22(6):880. doi: 10.3390/molecules22060880.
One of the most effective strategies to enhance metabolite biosynthesis and accumulation in biotechnological systems is the use of elicitation processes. This study assesses the influence of different concentrations of yeast extract (YE) on ginsenoside biosynthesis in (American ginseng) hairy roots cultivated in shake flasks and in a nutrient sprinkle bioreactor after 3 and 7 days of elicitation. The saponin content was determined using HPLC. The maximum yield (20 mg g d.w.) of the sum of six examined ginsenosides (Rb1, Rb2, Rc, Rd, Re and Rg1) in hairy roots cultivated in shake flasks was achieved after application of YE at 50 mg L concentration and 3 day exposure time. The ginsenoside level was 1.57 times higher than that attained in control medium. The same conditions of elicitation (3 day time of exposure and 50 mg L of YE) also favourably influenced the biosynthesis of studied saponins in bioreactor cultures. The total ginsenoside content was 32.25 mg g d.w. and was higher than that achieved in control medium and in shake flasks cultures. Obtained results indicated that yeast extract can be used to increase ginsenoside production in hairy root cultures of .
提高生物技术系统中代谢物生物合成和积累的最有效策略之一是使用诱导过程。本研究评估了不同浓度酵母提取物（YE）对在摇瓶中培养的西洋参毛状根以及在诱导3天和7天后的营养喷淋生物反应器中人参皂苷生物合成的影响。使用高效液相色谱法测定皂苷含量。在摇瓶中培养的毛状根中，当YE浓度为50 mg/L且暴露时间为3天时，六种检测的人参皂苷（Rb1、Rb2、Rc、Rd、Re和Rg1）总和的最大产量（20 mg/g干重）得以实现。人参皂苷水平比对照培养基中的高出1.57倍。相同的诱导条件（3天暴露时间和50 mg/L的YE）也对生物反应器培养物中所研究皂苷的生物合成产生了有利影响。总人参皂苷含量为32.25 mg/g干重，高于对照培养基和摇瓶培养中的含量。所得结果表明，酵母提取物可用于提高西洋参毛状根培养物中的人参皂苷产量。
