Smeriglio Antonella, Denaro Marcella, Barreca Davide, Calderaro Antonella, Bisignano Carlo, Ginestra Giovanna, Bellocco Ersilia, Trombetta Domenico
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, , Viale F. Stagno d'Alcontres 31, 98166 Messina, Italy.
Int J Mol Sci. 2017 Jun 6;18(6):1212. doi: 10.3390/ijms18061212.
Although the chemical composition and biological properties of some species of the genus has been investigated, studies on hull essential oil of L. variety Bronte (HEO) are currently lacking. In this work, we have carried out an in-depth phytochemical profile elucidation by Gas Chromatography-Mass Spectrometry (GC-MS) analysis, and an evaluation of antioxidant scavenging properties of HEO, using several different in vitro methods, checking also its cytoprotective potential on lymphocytes treated with tert-butyl hydroperoxide. Moreover, the antimicrobial activity against Gram-positive and Gram-negative strains, both American Type Culture Collection (ATCC) and clinical isolates, was also investigated. GC-MS analysis highlighted the richness of this complex matrix, with the identification of 40 derivatives. The major components identified were 4-Carene (31.743%), α-Pinene (23.584%), d-Limonene (8.002%), and 3-Carene (7.731%). The HEO showed a strong iron chelating activity and was found to be markedly active against hydroxyl radical, while scarce effects were found against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical. Moreover, pre-treatment with HEO was observed to significantly increase the cell viability, decreasing the lactate dehydrogenase (LDH) release. HEO was bactericidal against all the tested strains at the concentration of 7.11 mg/mL, with the exception of ATCC 9027. The obtained results demonstrate the strong free-radical scavenging activity of HEO along with remarkable cytoprotective and antimicrobial properties.
尽管已经对该属某些物种的化学成分和生物学特性进行了研究，但目前缺乏对勃朗特变种（HEO）船体精油的研究。在这项工作中，我们通过气相色谱 - 质谱联用（GC - MS）分析对其进行了深入的植物化学剖析，并使用几种不同的体外方法评估了HEO的抗氧化清除特性，还检测了其对叔丁基过氧化氢处理的淋巴细胞的细胞保护潜力。此外，还研究了其对革兰氏阳性和革兰氏阴性菌株（包括美国典型培养物保藏中心（ATCC）菌株和临床分离株）的抗菌活性。GC - MS分析突出了这种复杂基质的丰富性，鉴定出了40种衍生物。鉴定出的主要成分是4 - 蒈烯（31.743%）、α - 蒎烯（23.584%）、d - 柠檬烯（8.002%）和3 - 蒈烯（7.731%）。HEO表现出很强的铁螯合活性，对羟基自由基有显著活性，而对2,2 - 二苯基 - 1 - 苦基肼（DPPH）自由基的作用较弱。此外，观察到用HEO预处理可显著提高细胞活力，降低乳酸脱氢酶（LDH）释放。除了ATCC 9027外，HEO在浓度为7.11 mg/mL时对所有测试菌株均具有杀菌作用。所得结果表明HEO具有很强的自由基清除活性以及显著的细胞保护和抗菌特性。
