Songserm Pattralak, Klanrit Poramaporn, Klanrit Poramate, Phetcharaburanin Jutarop, Thanonkeo Pornthap, Apiraksakorn Jirawan, Phomphrai Khamphee, Klanrit Preekamol
Graduate School, Khon Kaen University, Khon Kaen 40002, Thailand.
Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand.
Plants (Basel). 2022 Jul 30;11(15):1994. doi: 10.3390/plants11151994.
The potential benefits of natural plant extracts have received attention in recent years, encouraging the development of natural products that effectively treat various diseases. This is the first report on establishing callus and cell suspension cultures of (L.) Kurz. A yellow friable callus was successfully induced from in vitro leaf explants on Murashige and Skoog medium supplemented with 1 mg/L 2,4-dichlorophenoxyacetic acid and 1 mg/L 1-naphthalene acetic acid. A selected friable callus line was used to establish the cell suspension culture with the same medium. The antioxidant assays showed that the leaf- and ethanolic-suspension-cultured cell (SCC) extracts exhibited high antioxidant potential. In addition, the in vitro cytotoxicity revealed by the MTT assay demonstrated potent antiproliferative effects against the oral cancer cell lines ORL-48 and ORL-136 in a dose-dependent manner. Several groups of compounds, including terpenoids, phenolics, flavonoids, quinones, and stilbenes, were identified by UHPLC-QToF-MS, with the same compounds detected in leaf and SCC extracts, including austroinulin, lucidenic acid, esculetin, embelin, and quercetin 3-(2″-p-hydroxybenzoyl-4″-p-coumarylrhamnoside). The present study suggests the value of further investigations for phytochemical production using cell suspension culture.
近年来，天然植物提取物的潜在益处受到关注，这推动了有效治疗各种疾病的天然产物的开发。这是关于建立（L.）库尔兹愈伤组织和细胞悬浮培养物的首篇报道。在添加了1毫克/升2,4 - 二氯苯氧乙酸和1毫克/升1 - 萘乙酸的Murashige和Skoog培养基上，成功从离体叶片外植体诱导出黄色易碎愈伤组织。选用一条易碎愈伤组织系，用相同培养基建立细胞悬浮培养物。抗氧化分析表明，叶片和乙醇悬浮培养细胞（SCC）提取物具有较高的抗氧化潜力。此外，MTT分析显示的体外细胞毒性表明，其对口腔癌细胞系ORL - 48和ORL - 136具有剂量依赖性的强效抗增殖作用。通过超高效液相色谱 - 四极杆飞行时间质谱（UHPLC - QToF - MS）鉴定出几类化合物，包括萜类、酚类、黄酮类、醌类和芪类，在叶片和SCC提取物中检测到相同的化合物，包括澳洲菊糖、灵芝酸、七叶亭、恩贝灵和槲皮素3 -（2″ - 对羟基苯甲酰基 - 4″ - 对香豆酰鼠李糖苷）。本研究表明进一步研究利用库尔兹细胞悬浮培养生产植物化学物质的价值。
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