Zhang Guangyuan, Duan Xiaohua, Zhang Chao, Chen Pu, Yu Jie, Zheng Jin
College of Chinese Materia Medica, Yunnan University of Traditional Chinese Medicine, Kunming, Yunnan 650500, P.R. China.
College of Ethnopharmacy, Yunnan University of Traditional Chinese Medicine, Kunming, Yunnan 650500, P.R. China.
Exp Ther Med. 2017 Jun;13(6):3175-3182. doi: 10.3892/etm.2017.4442. Epub 2017 May 8.
The Dai prescription Yajieshaba is widely used in Traditional Dai Medicine to treat food allergies and intolerance. However, information on the active chemical ingredients, effects and mechanisms of action of Yajieshaba is limited. The present study aimed to elucidate the effects and underlying mechanisms of Yajieshaba in the treatment of food allergies. Liquid chromatography with a diode array detector was used to measure the levels of palmatine and berberine, the active ingredients of Yajieshaba. A food allergy model was established in female BALB/c mice by three injections of ovalbumin (OVA) at 0, 48, and 96 h. OVA-sensitized mice recieved no treatments (control), Yajieshaba, loratadine, palmatine or berberine. The scratching frequency, serum immunoglobulin (Ig)G, IgE, interleukin (IL)-4, IL-10, IL-17, IL-21, interferon-γ and tumor necrosis factor-α levels were assessed at 50 and 98 h. The percentage of regulatory T cells (Tregs) was evaluated by flow cytometry at 98 h. The scratching frequency induced by OVA was significantly suppressed in mice treated with loratadine, palmatine, berberine or 3.50 and 4.70 g/kg Yajieshaba. The frequency of CD4CD25Treg in the spleen increased from 6.80% in mice in the control group to 12.50% in mice treated with 4.70 g/kg body weight Yajieshaba. Mice treated with palmatine or 4.70 g/kg body weight Yajieshaba had increased forkhead box p3 expression compared with those in the control group. Treatment with Yajieshaba decreased the scratching frequency and increased CD4CD25Foxp3 Treg frequency in the spleen. This indicated that symptoms of allergic reaction were alleviated following Yajieshaba treatment. Palmatine was identified as one of the major active components of Yajieshaba. The present study identified the possible mechanism through which Yajieshaba treatment may alleviate food allergy symptoms.
傣药方亚解沙巴在传统傣医中广泛用于治疗食物过敏和不耐受。然而，关于亚解沙巴的活性化学成分、作用效果及作用机制的信息有限。本研究旨在阐明亚解沙巴治疗食物过敏的效果及潜在机制。采用带二极管阵列检测器的液相色谱法测定亚解沙巴的活性成分巴马汀和小檗碱的含量。通过在0、48和96小时对雌性BALB/c小鼠进行三次卵清蛋白（OVA）注射建立食物过敏模型。OVA致敏小鼠未接受任何处理（对照组）、亚解沙巴、氯雷他定、巴马汀或小檗碱。在50和98小时评估抓挠频率、血清免疫球蛋白（Ig）G、IgE、白细胞介素（IL）-4、IL-10、IL-17、IL-21、干扰素-γ和肿瘤坏死因子-α水平。在98小时通过流式细胞术评估调节性T细胞（Tregs）的百分比。用氯雷他定、巴马汀、小檗碱或3.50和4.70 g/kg亚解沙巴处理的小鼠中，OVA诱导的抓挠频率显著降低。脾脏中CD4CD25Treg的频率从对照组小鼠的6.80%增加到用4.70 g/kg体重亚解沙巴处理的小鼠的12.50%。与对照组相比，用巴马汀或4.70 g/kg体重亚解沙巴处理的小鼠叉头框p3表达增加。用亚解沙巴处理可降低抓挠频率并增加脾脏中CD4CD25Foxp3 Treg频率。这表明亚解沙巴治疗后过敏反应症状得到缓解。巴马汀被确定为亚解沙巴的主要活性成分之一。本研究确定了亚解沙巴治疗可能缓解食物过敏症状的潜在机制。
