Ogiso-Tanaka Eri, Tanaka Tsuyoshi, Tanaka Keisuke, Nonoue Yasunori, Sasaki Takuji, Fushimi Erina, Koide Yohei, Okumoto Yutaka, Yano Masahiro, Saito Hiroki
National Institute of Agrobiological Sciences (NIAS), 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan.
Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo 156-8502, Japan.
Breed Sci. 2017 Mar;67(2):101-109. doi: 10.1270/jsbbs.16096. Epub 2017 Mar 4.
Heading date is one of the most important traits in rice breeding. It is governed by multiple genes, including known quantitative trait loci (QTLs). In general, almost all cultivars, including Nipponbare, head early under short-day (SD) conditions, but some cultivars, including Kasalath, head late. To explain this difference, we identified QTLs controlling heading date under SD conditions. We used NILs, CSSLs, and BILs from a cross between Nipponbare and Kasalath, and evaluated days to heading (DTH) under SD conditions. No NILs or CSSLs showed late heading, but two BILs (BIL-55 and BIL-78) had almost the same DTH as Kasalath. We developed an F population from a cross between BIL-55 and Nipponbare and performed QTL analysis using SSR markers. The late-heading phenotype was controlled by two known genes and at least two novel QTLs on chromosomes 4 and 6, named and . These QTLs were confirmed by QTL-seq. The QTLs and polymorphisms detected here will provide useful information for further genetic studies and breeding under SD conditions at lower latitudes.
抽穗期是水稻育种中最重要的性状之一。它受多个基因控制，包括已知的数量性状位点（QTL）。一般来说，几乎所有品种，包括日本晴，在短日照（SD）条件下抽穗早，但一些品种，包括卡萨拉思，抽穗晚。为了解释这种差异，我们鉴定了在SD条件下控制抽穗期的QTL。我们使用了来自日本晴和卡萨拉思杂交的近等基因系（NIL）、染色体片段代换系（CSSL）和染色体单片段代换系（BIL），并评估了SD条件下的抽穗天数（DTH）。没有NIL或CSSL表现出晚抽穗，但两个BIL（BIL-55和BIL-78）的DTH与卡萨拉思几乎相同。我们从BIL-55和日本晴的杂交后代中构建了一个F群体，并使用SSR标记进行QTL分析。晚抽穗表型由两个已知基因和位于第4和第6染色体上的至少两个新QTL控制，分别命名为 和 。这些QTL通过QTL-seq得到了验证。这里检测到的QTL和多态性将为低纬度地区SD条件下的进一步遗传研究和育种提供有用信息。
