Tian Chunyan, Huang Di, Yu Yanli, Zhang Jihong, Fang Qingxin, Xie Chao
Department of Health Care and Geriatrics, Yidu Central Hospital Affiliated to Weifang Medical University, Qingzhou, Shandong 262500, P.R. China.
Department of Clinical Medicine, Medical School of Shandong University, Jinan, Shandong 250012, P.R. China.
Exp Ther Med. 2017 Jun;13(6):3189-3194. doi: 10.3892/etm.2017.4393. Epub 2017 Apr 27.
ATP-binding cassette transporter G1 (ABCG1) is a member of the ABC transporter family and regulates cellular cholesterol homeostasis. It has important roles in cholesterol homeostasis and tumor immunity, which has, however, not been reported in lung cancer. The present study showed that ABCG1 protein is upregulated in lung cancer compared with adjacent normal tissues and furthermore, aberrant ABCG1 expression was detected in lung cancer cell lines. ABCG1 was shown to promote the proliferation of HKULC4 lung cancer cells. Moreover, ABCG1 was found to regulate proliferation-, apoptosis- and cancer stem cell-associated markers in HKULC4 cells, implying that ABCG1 has important roles not only in the regulation of proliferation but also of apoptosis and cancer stem cells. A microRNA microarray analysis showed that ABCG1 significantly downregulated miR-29a, -b and -c expression in HKULC4 cells. Finally, it was demonstrated that ABCG1 promoted the migration and invasion in HKULC4 cells. Thus, ABCG1 may be a novel therapeutic target to improve the treatment of non-small cell lung cancer.
ATP结合盒转运体G1（ABCG1）是ABC转运体家族的成员，可调节细胞胆固醇稳态。它在胆固醇稳态和肿瘤免疫中发挥重要作用，然而，在肺癌中尚未见相关报道。本研究表明，与相邻正常组织相比，ABCG1蛋白在肺癌中上调，此外，在肺癌细胞系中检测到ABCG1表达异常。结果显示ABCG1可促进HKULC4肺癌细胞的增殖。此外，发现ABCG1可调节HKULC4细胞中与增殖、凋亡和癌症干细胞相关的标志物，这意味着ABCG1不仅在增殖调节中起重要作用，而且在凋亡和癌症干细胞调节中也起重要作用。微小RNA微阵列分析表明，ABCG1显著下调HKULC4细胞中miR-29a、-b和-c的表达。最后，证明ABCG1促进HKULC4细胞的迁移和侵袭。因此，ABCG1可能是改善非小细胞肺癌治疗的新型治疗靶点。
