Cui Hairui, Wu Qiongyu, Zhu Bin
Institute of Nuclear-Agricultural Sciences/Key Laboratory of Chinese Ministry of Agriculture for Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou 310029, China.
Biomed Res Int. 2017;2017:4816973. doi: 10.1155/2017/4816973. Epub 2017 May 15.
Genomic stability depends in part on an efficient DNA lesion recognition and correction by the DNA mismatch repair (MMR) system. We investigated mutations arising spontaneously in rice mutants by specific-locus amplified fragment sequencing. Totally 994 single-nucleotide mutations were identified in three mutants and on average the mutation density is about 1/136.72 Kb per mutant line. These mutations were relatively randomly distributed in genome and might be accumulated in generation-dependent manner. All possible base transitions and base transversions could be seen and the ratio of transitions to transversions was about 3.12. We also observed the nearest-neighbor bias around the mutated base. Our data suggests that is important in ensuring genome stability by recognizing mismatches that arise spontaneously and provides useful information for investigating the function of the gene in DNA repair and exploiting MMR mutants in rice induced mutation breeding.
基因组稳定性部分取决于DNA错配修复（MMR）系统对DNA损伤的有效识别和校正。我们通过特定位点扩增片段测序研究了水稻突变体中自发产生的突变。在三个突变体中总共鉴定出994个单核苷酸突变，每个突变系的平均突变密度约为1/136.72 Kb。这些突变在基因组中相对随机分布，并且可能以世代依赖的方式积累。可以看到所有可能的碱基转换和碱基颠换，转换与颠换的比例约为3.12。我们还观察到突变碱基周围的近邻偏好。我们的数据表明，通过识别自发产生的错配， 对于确保基因组稳定性很重要，并为研究 基因在DNA修复中的功能以及在水稻诱变育种中利用MMR突变体提供了有用的信息。
