Jiang Meng, Wu Xiaojiang, Song Yue, Shen Hongzhe, Cui Hairui
Key Laboratory of Chinese Ministry of Agriculture for Nuclear-Agricultural Sciences, Institute of Nuclear-Agricultural Science, Zhejiang University, Hangzhou, China.
National Key Laboratory of Rice Biology, Institute of Crop Sciences, Zhejiang University, Hangzhou, China.
Front Plant Sci. 2020 Mar 3;11:220. doi: 10.3389/fpls.2020.00220. eCollection 2020.
DNA mismatch repair (MMR) system is important for maintaining DNA replication fidelity and genome stability by repairing erroneous deletions, insertions and mis-incorporation of bases. With the aim of deciphering the role of the MMR system in genome stability and recombination in rice, we investigated the function of gene, an import component of the MMR system. To achieve this goal, homeologous recombination and endogenous microsatellite stability were evaluated by using rice mutants carrying a insertion into the gene. Totally 60 microsatellites were analyzed and 15 distributed on chromosome 3, 6, 8, and 10 showed instability in three mutants, D6011, NF7784 and NF9010, compared with the wild type MSH6WT (the control). The disruption of gene is associated with modest increases in homeologous recombination, ranging from 2.0% to 32.5% on chromosome 1, 3, 9, and 10 in the BCF populations of the mutant ND6011 and NF9010. Our results suggest that the plays an important role in ensuring genome stability and genetic recombination, providing the first evidence for the gene in maintaining microsatellite stability and restricting homeologous recombination in plants.
DNA错配修复（MMR）系统对于通过修复错误的缺失、插入和碱基错掺入来维持DNA复制保真度和基因组稳定性至关重要。为了解析MMR系统在水稻基因组稳定性和重组中的作用，我们研究了MMR系统的一个重要组成部分—— 基因的功能。为实现这一目标，我们利用携带 基因插入的水稻突变体评估了同源重组和内源性微卫星稳定性。总共分析了60个微卫星，其中分布在第3、6、8和10号染色体上的15个微卫星在三个突变体D6011、NF7784和NF9010中与野生型MSH6WT（对照）相比表现出不稳定性。 基因的破坏与同源重组的适度增加有关，在突变体ND6011和NF9010的BCF群体中，第1、3、9和10号染色体上的同源重组增加幅度在2.0%至32.5%之间。我们的结果表明， 在确保基因组稳定性和遗传重组方面发挥着重要作用，为该基因在维持植物微卫星稳定性和限制同源重组方面提供了首个证据。
