Mondal Nandan K, Siddique Shabana, Banerjee Madhuchanda, Roychoudhury Sanghita, Mukherjee Sayali, Slaughter Mark S, Lahiri Twisha, Ray Manas R
Department of Experimental Hematology, Chittaranjan National Cancer Institute, Kolkata, India.
Department of Cardiovascular and Thoracic Surgery, Cardiovascular Innovation Institute, University of Louisville School of Medicine, Louisville, KY, USA.
Saf Health Work. 2017 Jun;8(2):198-205. doi: 10.1016/j.shaw.2016.10.004. Epub 2016 Dec 9.
There are a million ragpickers in India who gather and trade recyclable municipal solid wastes materials for a living. The objective of this study was to examine whether their occupation adversely affects their immunity.
Seventy-four women ragpickers (median age, 30 years) and 65 age-matched control housemaids were enrolled. Flow cytometry was used to measure leukocyte subsets, and leukocyte expressions of Fcγ receptor I (CD64), FcγRIII (CD16), complement receptor 1 (CD35) and CR3 (CD11b/CD18), and CD14. Serum total immunoglobulin-E was estimated with enzyme-linked immunosorbent assay.
Compared with the controls, ragpickers had significantly ( < 0.0001) higher levels of CD8+T-cytotoxic, CD16+CD56+natural killer, and CD4+CD45RO+memory T-cells, but depleted levels of CD19+B-cells. The percentage of CD4+T-helper-cells was lower than the control group ( < 0.0001), but their absolute number was relatively unchanged ( = 0.42) due to 11% higher lymphocyte counts in ragpickers. In ragpickers, the percentages of CD14+CD16+intermediate and CD14dim CD16+nonclassical monocyte subsets were elevated with a decline in CD14+CD16-classical monocytes. The expressions of CD64, CD16, CD35, and CD11b/CD18 on both monocytes and neutrophils, and CD14 on monocytes were significantly higher in ragpickers. In addition, ragpickers had 2.7-times more serum immunoglobulin-E than the controls ( < 0.0001). After controlling potential confounders, the profession of ragpicking was positively associated with the changes.
Ragpicking is associated with alterations in both innate (neutrophils, monocytes, and natural killer cell numbers and expression of complement and Fcγ receptors) and adaptive immunity (numbers of circulating B cells, helper, cytotoxic, and memory T cells).
印度有100万拾荒者，他们靠收集和交易可回收的城市固体废弃物为生。本研究的目的是检验他们的职业是否会对其免疫力产生不利影响。
招募了74名女性拾荒者（中位年龄30岁）和65名年龄匹配的对照家庭佣工。采用流式细胞术检测白细胞亚群、Fcγ受体I（CD64）、FcγRIII（CD16）、补体受体1（CD35）和CR3（CD11b/CD18）以及CD14的白细胞表达。用酶联免疫吸附测定法估算血清总免疫球蛋白E。
与对照组相比，拾荒者的CD8 + T细胞毒性细胞、CD16 + CD56 +自然杀伤细胞和CD4 + CD45RO +记忆T细胞水平显著升高（<0.0001），但CD19 + B细胞水平降低。CD4 +辅助性T细胞的百分比低于对照组（<0.0001），但由于拾荒者的淋巴细胞计数高11%，其绝对数量相对未变（=0.42）。在拾荒者中，CD14 + CD16 +中间型和CD14dim CD16 +非经典单核细胞亚群的百分比升高，而CD14 + CD16 -经典单核细胞减少。拾荒者单核细胞和中性粒细胞上CD64、CD16、CD35和CD11b/CD18的表达以及单核细胞上CD14的表达均显著更高。此外，拾荒者的血清免疫球蛋白E比对照组多2.7倍（<0.0001）。在控制潜在混杂因素后，拾荒职业与这些变化呈正相关。
拾荒与固有免疫（中性粒细胞、单核细胞和自然杀伤细胞数量以及补体和Fcγ受体的表达）和适应性免疫（循环B细胞、辅助性、细胞毒性和记忆T细胞数量）的改变有关。
