Laboratorio de Biología Molecular e Inmunología, Centro Universitario del Sur, Universidad de Guadalajara, Av. Enrique Arreola Silva No. 883, 49000. Cd Guzmán, Jalisco México.
División de Inmunología, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Sierra Mojada No. 800, 44340 Guadalajara, Jalisco México.
Cancer Cell Int. 2014 Sep 24;14(1):97. doi: 10.1186/s12935-014-0097-5. eCollection 2014.
In response to antigen naive CD8+, T cells differentiate into effector cells, which express Natural killer (NK) receptors, lose CD28 expression, and die by apoptosis. However, in smaller quantities, the cells are retained for subsequent exposure to the same antigen. Knowledge is limited regarding whether the percentages of CD28-, Effector memory (EMRA(null/dim)), and the CD16+/CD56 + CD8+ T cells of women with low-grade cervical lesions are altered at a systemic level.
We enrolled in this study women controls and women with Human papilloma virus infection (HPV-I) without associated cellular neoplastic changes and with Cervical Intraepithelial Neoplastic-I (CIN-I). Flow cytometry (FC) was performed for measurement of CD28-, memory subset, and NK-like CD8 + T cells, and IL-17, IFN-gamma, Tumor necrosis factor (TNF)-alpha, Interleukin (IL)-10, IL-6, IL-4, and IL-2. Finally, we genotyped the HPV.
The CIN-I group increased the CD8 + CD28- and CD16+/56+ T cell percentage compared with that of HPV-I and controls (p <0.01), and CD8 + CCR7-CD45RA(null/dim) (EMRA(null/dim)) T cells were also increased in the CIN-I group compared with the controls (p <0.01). These two study groups were HPV- genotyped; 49% were HPV18+, and we did not observe differences in cytokine levels among all groups.
Increased levels of CD28-, EMRA(null/dim), and CD16+/CD56 + CD8+ T cells of peripheral blood in women with CIN-I may be associated with persistent HPV infection and could exert an influence on progression to cervical cancer.
针对抗原幼稚 CD8+T 细胞，分化为效应细胞，表达自然杀伤（NK）受体，失去 CD28 表达，并通过细胞凋亡死亡。然而，在较小的数量中，细胞被保留用于随后暴露于相同的抗原。关于低度宫颈病变女性的 CD28-、效应记忆（EMRA(null/dim)）和 CD16+/CD56+CD8+T 细胞的百分比是否在系统水平上发生改变，目前知识有限。
我们招募了本研究中的女性对照组和 HPV 感染（HPV-I）的女性，且没有相关的细胞肿瘤性改变，以及宫颈上皮内瘤变-I（CIN-I）。流式细胞术（FC）用于测量 CD28-、记忆亚群和 NK 样 CD8+T 细胞以及 IL-17、IFN-γ、肿瘤坏死因子（TNF）-α、白细胞介素（IL）-10、IL-6、IL-4 和 IL-2。最后，我们对 HPV 进行了基因分型。
CIN-I 组与 HPV-I 组和对照组相比，CD8+CD28-和 CD16+/56+T 细胞百分比增加（p<0.01），并且 CIN-I 组的 CD8+CCR7-CD45RA(null/dim)（EMRA(null/dim)）T 细胞也增加与对照组相比（p<0.01）。这两个研究组进行了 HPV 基因分型；49%为 HPV18+，我们没有观察到所有组之间细胞因子水平的差异。
CIN-I 女性外周血中 CD28-、EMRA(null/dim)和 CD16+/CD56+CD8+T 细胞水平升高可能与持续的 HPV 感染有关，并可能对宫颈癌的进展产生影响。
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