Toll 样细胞受体 TLR2（p.Arg753GLN）和 TLR4（p.Asp299GLY）多态性与特应性皮炎患者全身和局部免疫指标的关系。
Association of Toll-Like Cell Receptors TLR2 (p.Arg753GLN) and TLR4 (p.Asp299GLY) Polymorphisms with Indicators of General and Local Immunity in Patients with Atopic Dermatitis.
机构信息
Department of Biochemistry and Clinical Laboratory Diagnostics, Kazan State Medical University, 49 Butlerov Street, Kazan 420012, Russia.
Laboratory of Immunology and the Development of Allergens, Kazan Research Institute of Epidemiology and Microbiology, 67 Big Red Street, Kazan 420015, Russia.
出版信息
J Immunol Res. 2017;2017:8493545. doi: 10.1155/2017/8493545. Epub 2017 May 16.
A whole group of polymorphisms of genes involved in the formation of the epidermal barrier, immune responses, and their regulation is important in the formation of atopic phenotype. The purpose of the study is to determine the relationship of polymorphisms of genes of Toll-like receptors TLR2 and TLR4 with clinical and immunological parameters in atopic dermatitis patients in a "case-control" study. Polymorphisms of genes TLR2 (p.Arg753Gln) and TLR4 (Asp299Gly) were detected by PCR. Parameters of the state of innate and adaptive immunity were assessed by the level of local production of sIgA, cytokine profile of blood serum for IL-4, IL-10, and IFN-. Biological samples from 50 people with allergic pathology, aged 4.5 to 35 years, and 100 healthy individuals (controls) were analyzed. Observed dysregulation of cytokine production (IL-4, IL-10) in patients with heterozygous polymorphic genotypes probably reflects an imbalance of Th1/Th2/Th17 regulation of immune system response in these individuals.
一整组参与表皮屏障形成、免疫反应及其调节的基因多态性在特应性表型的形成中很重要。本研究的目的是在一项“病例对照”研究中确定 Toll 样受体 TLR2 和 TLR4 基因多态性与特应性皮炎患者临床和免疫学参数的关系。通过 PCR 检测 TLR2（p.Arg753Gln）和 TLR4（Asp299Gly）基因的多态性。通过局部产生 sIgA 的水平、血清细胞因子谱评估固有和适应性免疫状态，用于 IL-4、IL-10 和 IFN-。分析了 50 名年龄在 4.5 至 35 岁之间的过敏病理患者和 100 名健康个体（对照）的生物样本。在杂合多态基因型的患者中观察到细胞因子产生（IL-4、IL-10）的失调可能反映了这些个体的免疫系统反应中 Th1/Th2/Th17 调节的失衡。
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