Kato Kumiko, Odamaki Toshitaka, Mitsuyama Eri, Sugahara Hirosuke, Xiao Jin-Zhong, Osawa Ro
Next Generation Science Institute R&D Division, Morinaga Milk Industry Co., Ltd., 5-1-83, Higashihara, Zama-city, Kanagawa, 252-8583, Japan.
Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo, Japan.
Curr Microbiol. 2017 Aug;74(8):987-995. doi: 10.1007/s00284-017-1272-4. Epub 2017 Jun 8.
Bifidobacteria are one of the major components in human microbiota that are suggested to function in maintaining human health. The colonization and cell number of Bifidobacterium species in human intestine vary with ageing. However, sequential changes of Bifidobacterium species ranging from newborns to centenarians remain unresolved. Here, we investigated the gut compositional changes of Bifidobacterium species over a wide range of ages. Faecal samples of 441 healthy Japanese subjects between the ages of 0 and 104 years were analysed using real-time PCR with species-specific primers. B. longum group was widely detected from newborns to centenarians, with the highest detection rate. B. breve was detected in approximately 70% of children under 3 years old. B. adolescentis and B. catenulatum groups were predominant after weaning. B. bifidum was detected at almost all ages. The detection rate of B. dentium was higher in the elderly than in other ages. B. animalis ssp. lactis was detected in 11.4% of the subjects and their ages were restricted. B. gallinarum goup was detected in only nine subjects, while B. minimum and B. mongoliense were undetected at any age. The presence of certain Bifidobacterium groups was associated with significantly higher numbers of other Bifidobacterium species/subspecies. Inter-species correlations were found among each species, exception for B. animalis ssp. lactis. These results revealed the patterns and transition points with respect to compositional changes of Bifidobacterium species that occur with ageing, and the findings indicate that there may be symbiotic associations between some of these species in the gut microbiota.
双歧杆菌是人类微生物群的主要组成部分之一，被认为在维持人类健康方面发挥作用。双歧杆菌在人类肠道中的定植和细胞数量会随着年龄的增长而变化。然而，从新生儿到百岁老人双歧杆菌种类的连续变化仍未得到解决。在这里，我们研究了广泛年龄段双歧杆菌种类的肠道组成变化。使用物种特异性引物通过实时PCR分析了441名年龄在0至104岁之间的健康日本受试者的粪便样本。从新生儿到百岁老人都广泛检测到长双歧杆菌组，检测率最高。在大约70%的3岁以下儿童中检测到短双歧杆菌。断奶后青春双歧杆菌和链状双歧杆菌组占主导地位。几乎在所有年龄段都检测到了两歧双歧杆菌。老年人口腔双歧杆菌的检出率高于其他年龄段。在11.4%的受试者中检测到动物双歧杆菌亚种乳酸亚种，且其年龄受限。仅在9名受试者中检测到鸡双歧杆菌组，而在任何年龄都未检测到极小双歧杆菌和蒙古双歧杆菌。某些双歧杆菌组的存在与其他双歧杆菌种类/亚种数量的显著增加相关。除动物双歧杆菌亚种乳酸亚种外，在每个物种之间都发现了种间相关性。这些结果揭示了随着年龄增长双歧杆菌种类组成变化的模式和转变点，研究结果表明肠道微生物群中的某些物种之间可能存在共生关系。
