Probiotics Research Laboratory, Juntendo University Graduate School of Medicine, Hongo 2-9-8-3F, Bunkyo-ku, Tokyo, 113-0033, Japan.
Gut Microbiome and Metabolic Diseases, Center for Diabetes, Obesity and Metabolism, Wake Forest School of Medicine, Biotech Place, Winston-Salem, NC, 27101, USA.
Sci Rep. 2017 Aug 30;7(1):10097. doi: 10.1038/s41598-017-10711-5.
Bifidobacteria are important members of human gut microbiota; however, quantitative data on their early-life dynamics is limited. Here, using a sensitive reverse transcription-qPCR approach, we demonstrate the carriage of eight signature infant-associated Bifidobacterium species (B. longum, B. breve, B. bifidum, B. catenulatum group, B. infantis, B. adolescentis, B. angulatum and B. dentium) in 76 healthy full-term vaginally-born infants from first day to three years of life. About 21% babies carry bifidobacteria at first day of life (6.2 ± 1.9 log cells/g feces); and this carriage increases to 64% (8.0 ± 2.2), 79% (8.5 ± 2.1), 97% (9.3 ± 1.8), 99% (9.6 ± 1.6), and 100% (9.7 ± 0.9) at age 7 days, 1, 3 and 6 months, and 3 years, respectively. B. longum, B. breve, B. catenulatum group and B. bifidum are among the earliest and abundant bifidobacterial clades. Interestingly, infants starting formula-feed as early as first week of life have higher bifidobacterial carriage compared to exclusively breast-fed counterparts. Bifidobacteria demonstrate an antagonistic correlation with enterobacteria and enterococci. Further analyses also reveal a relatively lower/ delayed bifidobacterial carriage in cesarean-born babies. The study presents a quantitative perspective of the early-life gut Bifidobacterium colonization and shows how factors such as birth and feeding modes could influence this acquisition even in healthy infants.
双歧杆菌是人类肠道菌群的重要成员；然而，其早期生命动态的定量数据有限。在这里，我们使用一种敏感的反转录 qPCR 方法，在 76 名健康足月阴道分娩的婴儿中，从出生第一天到三岁，证明了八种特征婴儿相关双歧杆菌物种（长双歧杆菌、短双歧杆菌、双歧双歧杆菌、双歧双歧杆菌、婴儿双歧杆菌、双歧双歧杆菌、双歧双歧杆菌和双歧双歧杆菌）的携带情况。约 21%的婴儿在出生第一天携带双歧杆菌（粪便中 6.2±1.9log 细胞/g）；这种携带量增加到 64%（8.0±2.2）、79%（8.5±2.1）、97%（9.3±1.8）、99%（9.6±1.6）和 100%（9.7±0.9）在 7 天、1 天、3 个月和 6 个月和 3 岁时。长双歧杆菌、短双歧杆菌、双歧双歧杆菌和双歧双歧杆菌是最早和丰富的双歧杆菌分支之一。有趣的是，早在出生后第一周就开始配方喂养的婴儿，其双歧杆菌携带量高于纯母乳喂养的婴儿。双歧杆菌与肠杆菌和肠球菌呈拮抗相关性。进一步的分析还表明，剖宫产婴儿的双歧杆菌携带量较低/延迟。该研究提供了早期生命肠道双歧杆菌定植的定量视角，并展示了出生和喂养方式等因素如何影响这种定植，即使是在健康婴儿中。
