Zhang Zecai, Li Yanxin, Shen Peng, Li Shan, Lu Xiaojie, Liu Jiuxi, Cao Yongguo, Liu Bo, Fu Yunhe, Zhang Naisheng
College of Veterinary Medicine, Jilin University, Changchun 130062, People's Republic of China.
College of Veterinary Medicine, Jilin University, Changchun 130062, People's Republic of China.
Int Immunopharmacol. 2017 Aug;49:168-177. doi: 10.1016/j.intimp.2017.05.033. Epub 2017 Jun 5.
Ulcerative colitis (UC), an idiopathic inflammatory bowel disease, not only affects millions of patients worldwide, but also increases the risk of colon cancer. Geniposide is an iridoid glycoside and has many biological activities such as anti-inflammatory and antioxidant. However, its protective efficacy and mechanism of action against UC are still unclear. In this study, we aimed to investigate the protective effects and mechanisms of geniposide on dextran sulfate sodium (DSS)-induced experimental colitis in mice. The results revealed that geniposide alleviated body weight loss, disease activity index, colon length shortening and colonic pathological damage induced by DSS. Geniposide significantly suppressed pro-inflammatory cytokines by regulating NF-κB and PPARγ pathways in vivo and in vitro. Furthermore, geniposide also significantly regulated the expressions of ZO-1 and occludin in DSS-induced experimental colitis in mice and lipopolysaccharide (LPS)-triggered inflammation in Caco-2 cells. These findings indicated that geniposide may be a new natural chemopreventive agent to combat UC.
溃疡性结肠炎（UC）是一种特发性炎症性肠病，不仅影响着全球数百万患者，还会增加患结肠癌的风险。栀子苷是一种环烯醚萜苷，具有抗炎、抗氧化等多种生物活性。然而，其对UC的保护作用及其作用机制仍不清楚。在本研究中，我们旨在探讨栀子苷对葡聚糖硫酸钠（DSS）诱导的小鼠实验性结肠炎的保护作用及其机制。结果显示，栀子苷可减轻DSS诱导的体重减轻、疾病活动指数、结肠长度缩短及结肠病理损伤。栀子苷在体内和体外通过调节NF-κB和PPARγ通路显著抑制促炎细胞因子。此外，栀子苷还显著调节了DSS诱导的小鼠实验性结肠炎中ZO-1和闭合蛋白的表达以及脂多糖（LPS）引发的Caco-2细胞炎症。这些发现表明，栀子苷可能是一种对抗UC的新型天然化学预防剂。
