Sun Fangfang, Oh Sangjin, Kim Jeonghyo, Kato Tatsuya, Kim Hwa-Jung, Lee Jaebeom, Park Enoch Y
Research Institute of Green Science and Technology, Shizuoka University, Suruga-ku, Shizuoka 422-8529, Japan.
Department of Biomedical Engineering, College of Life Information Science and Instrument Engineering, Hangzhou Dianzi University, Hangzhou 310018, P.R. China.
J Microbiol Biotechnol. 2017 Aug 28;27(8):1483-1490. doi: 10.4014/jmb.1612.12041.
In this study, silver nanoparticles (AgNPs) were synthesized by the citrate reduction process and, with the assistance of -hydroxysuccinimide and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide, were successfully loaded with the macromolecular drug vancomycin (VAM) to form AgNP-VAM bioconjugates. The synthesized AgNPs, VAM, and AgNP-VAM conjugate were characterized by UV-visible spectroscopy, zeta potential analysis, confocal microscopy, and transmission electron microscopy. The effect of loading VAM onto AgNPs was investigated by testing the internalization of the bioconjugate into . After treatment with the AgNP-VAM conjugate, the bacterial cells showed a significant decrease in UV absorption, indicating that loading of the VAM on AgNPs had vastly improved the drug's internalization compared with that of AgNPs. All the experimental assessments showed that, compared with free AgNPs and VAM, enhanced internalization had been successfully achieved with the AgNP-VAM conjugate, thus leading to significantly better delivery of the macromolecular drug into the cell. The current research provides a new potential drug delivery system for the treatment of mycobacterial infections..
在本研究中，通过柠檬酸盐还原法合成了银纳米颗粒（AgNPs），并在N-羟基琥珀酰亚胺和1-乙基-3-(3-二甲基氨基丙基)碳二亚胺的辅助下，成功负载了大分子药物万古霉素（VAM），形成了AgNP-VAM生物共轭物。通过紫外-可见光谱、zeta电位分析、共聚焦显微镜和透射电子显微镜对合成的AgNPs、VAM和AgNP-VAM共轭物进行了表征。通过测试生物共轭物进入细胞的内化作用，研究了将VAM负载到AgNPs上的效果。用AgNP-VAM共轭物处理后，细菌细胞的紫外吸收显著降低，这表明与AgNPs相比，VAM负载到AgNPs上极大地提高了药物的内化作用。所有实验评估表明，与游离的AgNPs和VAM相比，AgNP-VAM共轭物成功实现了增强的内化作用，从而使大分子药物向细胞的递送明显更好。目前的研究为治疗分枝杆菌感染提供了一种新的潜在药物递送系统。
