Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences and Center of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok, Thailand.
Engineering and Cutaneous Biology Laboratory, INSERM UMR 1098, University of Franche-Comte, Besancon, France.
Photochem Photobiol. 2017 Nov;93(6):1462-1471. doi: 10.1111/php.12801. Epub 2017 Jul 25.
The current method for efficient evaluation of antiphotoaging compounds is an in vitro skin culture model using a single ultraviolet A (UVA) irradiation of fibroblasts. However, skin photoaging is caused by repeated exposure to UVA radiation. The objective of this study was to develop an appropriate model for in vitro skin photoaging by comparing the different effects of single (5 J cm ) and repeated exposures (5 J cm × 3 times) of fibroblasts to UVA irradiation. Our results demonstrated that a single and repeated exposure to UVA irradiation had different effects on fibroblasts. In the single UVA-irradiated group, collagen lattice contraction and the protein levels of type I procollagen and matrix metalloproteinase-1 (MMP-1) increased, while the levels of fibronectin and alpha-smooth muscle actin (α-SMA) were unchanged, compared to levels in the non-UVA-irradiated group (control). In contrast, repeated UVA exposure significantly induced G0/G1 cell cycle arrest, reduced collagen lattice contraction and type I procollagen and fibronectin expression, and increased MMP-1 expression. There was no difference in α-SMA expression when comparing repeatedly irradiated and non-UVA-irradiated fibroblasts. Our findings clearly indicate that repeated UVA irradiation of cells induces malfunctions found in photoaged skin and is an appropriate in vitro skin model of photoaging.
目前评估抗光老化化合物的有效方法是利用成纤维细胞进行单次紫外线 A（UVA）照射的体外皮肤培养模型。然而，皮肤光老化是由反复暴露于 UVA 辐射引起的。本研究旨在通过比较成纤维细胞单次（5 J cm ）和重复（5 J cm × 3 次）暴露于 UVA 辐射的不同影响，建立合适的体外皮肤光老化模型。我们的结果表明，单次和重复暴露于 UVA 辐射对成纤维细胞有不同的影响。在单次 UVA 照射组中，与未照射 UVA 的对照组相比，胶原晶格收缩以及 I 型前胶原和基质金属蛋白酶-1（MMP-1）的蛋白水平增加，而纤连蛋白和α-平滑肌肌动蛋白（α-SMA）的水平不变。相比之下，重复 UVA 暴露显著诱导 G0/G1 细胞周期停滞，降低胶原晶格收缩以及 I 型前胶原和纤连蛋白的表达，并增加 MMP-1 的表达。与非 UVA 照射的成纤维细胞相比，α-SMA 的表达没有差异。我们的研究结果清楚地表明，细胞的重复 UVA 照射会引起光老化皮肤中发现的功能障碍，是光老化的合适体外皮肤模型。
