Tvarijonaviciute Asta, Barranco Tomas, Rubio Monica, Carrillo Jose Maria, Martinez-Subiela Silvia, Tecles Fernando, Carrillo Juana Dolores, Cerón José J
Animal Medicine and Surgery Department, School of Veterinary Medicine, University of Murcia, Campus of Espinardo s/n, 30100, Murcia, Spain.
Animal Medicine and Surgery Department, School of Veterinary Medicine, Universidad CEU Cardenal Herrera of Valencia, Valencia, Spain.
BMC Vet Res. 2017 Jun 9;13(1):168. doi: 10.1186/s12917-017-1080-x.
Muscle enzymes in saliva have been reported to be possible markers of heart and muscle damage in humans. The aim of this study was to assess if Creatine kinase (CK) and Aspartate aminotransferase (AST) activities could be measured in canine saliva, and to evaluate their possible changes in situations of muscle damage.
The spectrophotometric assays for CK and AST measurement in saliva of dogs showed intra- and inter-assay imprecision lower than 1 and 16% and coefficients of correlation close to 1 in linearity under dilution tests. Healthy dogs showed activities in saliva of CK between 27 and 121 U/L and AST between 46 and 144 U/L, whereas in saliva of dogs with muscle damage CK ranged between 132 and 3862 U/L and AST between 154 and 4340 U/L. Positive moderate correlations were found between saliva and serum activities of the two enzymes (CK, r = 0.579; P = 0.001; AST, r = 0.674; P = 0.001).
CK and AST activities can be measured in canine saliva with commercially available spectrophotometric assays. In addition these enzymes show higher values in saliva of dogs with muscle damage and their values are moderately correlated with those of serum.
唾液中的肌肉酶据报道可能是人类心脏和肌肉损伤的标志物。本研究的目的是评估犬类唾液中是否可以检测到肌酸激酶（CK）和天冬氨酸氨基转移酶（AST）的活性，并评估它们在肌肉损伤情况下可能发生的变化。
犬类唾液中CK和AST测量的分光光度法检测显示，批内和批间不精密度低于1%和16%，在稀释试验下线性关系中的相关系数接近1。健康犬的唾液中CK活性在27至121 U/L之间，AST活性在46至144 U/L之间，而在肌肉损伤犬的唾液中，CK在132至3862 U/L之间，AST在154至4340 U/L之间。两种酶的唾液和血清活性之间存在中度正相关（CK，r = 0.579；P = 0.001；AST，r = 0.674；P = 0.001）。
使用市售分光光度法可以检测犬类唾液中的CK和AST活性。此外，这些酶在肌肉损伤犬的唾液中显示出较高的值，并且它们的值与血清值呈中度相关。
