Gueldner Jennifer, Zhang Fan, Zechmann Bernd, Bruce Erica D
Baylor University, Institute of Biomedical Studies, One Bear Place #97266, Waco, TX. 76798, United States.
Baylor University, Department of Environmental Science, One Bear Place #97266, Waco, TX 76798, United States.
Toxicol In Vitro. 2017 Sep;43:62-68. doi: 10.1016/j.tiv.2017.06.005. Epub 2017 Jun 7.
Non-gaseous oxygen therapeutics are emerging technologies in regenerative medicine that aim to sidestep the undesirable effects seen in traditional oxygen therapies, while enhancing tissue and wound regeneration. Using a novel oxygenating therapeutic (Ox66™) several in vitro models including fibroblast and keratinocyte monocultures were evaluated for potential drug toxicity, the ability of cells to recover after chemical injury, and cell migration after scratch assay. It was determined that in both cell lines, there was no significant cytotoxicity found after independent treatment with Ox66™. Similarly, after DMSO-induced chemical injury, the health parameters of cells treated with Ox66™ were improved when compared to their untreated counterparts. Particles were also characterized using scanning electron microscopy and electron dispersive spectroscopy both individually and in conjunction with fibroblast growth. The data in this study showed that the novel wound healing therapeutic has potential in advancing the treatment of various types of acute and chronic wounds.
非气态氧疗法是再生医学中的新兴技术，旨在规避传统氧疗法中出现的不良影响，同时促进组织和伤口再生。使用一种新型的氧合疗法（Ox66™），对包括成纤维细胞和角质形成细胞单培养物在内的几种体外模型进行了评估，以检测潜在的药物毒性、化学损伤后细胞的恢复能力以及划痕试验后的细胞迁移情况。结果表明，在两种细胞系中，单独用Ox66™处理后均未发现明显的细胞毒性。同样，在二甲基亚砜诱导的化学损伤后，与未处理的细胞相比，用Ox66™处理的细胞的健康参数得到了改善。还分别使用扫描电子显微镜和电子能谱对颗粒进行了表征，并结合成纤维细胞生长进行了分析。本研究中的数据表明，这种新型伤口愈合疗法在推进各类急慢性伤口的治疗方面具有潜力。
