Gunawardene Piumali, Al Saedi Ahmed, Singh Lakshman, Bermeo Sandra, Vogrin Sara, Phu Steven, Suriyaarachchi Pushpa, Pignolo Robert J, Duque Gustavo
Sydney Medical School Nepean, The University of Sydney, Penrith, NSW, Australia; Department of Geriatric Medicine, Nepean Hospital, Penrith, NSW, Australia.
Australian Institute for Musculoskeletal Science (AIMSS), The University of Melbourne and Western Health, Melbourne, VIC, Australia; Department of Medicine-Western Health, Melbourne Medical School, The University of Melbourne, Melbourne, VIC, Australia.
Exp Gerontol. 2017 Oct 1;96:68-72. doi: 10.1016/j.exger.2017.06.004. Epub 2017 Jun 6.
Circulating osteoprogenitor (COP) cells are blood-borne cells which express a variety of osteoblastic markers and are able to form bone nodules in vivo. Whereas a high percentage of COP cells (%COP) is associated with vascular calcification, low %COP has been associated with disability and frailty. However, the reference range of %COP in age- and gender-matching populations, and the age-related changes in %COP remain unknown. A cross-sectional study was undertaken in 144 healthy volunteers in Western Sydney (20-90year-old, 10 male and 10 female subjects per decade). %COP was quantified by flow cytometry. A high inter-and intra-rater reliability was found. In average, in this healthy population average of %COP was 0.42. There was no significant difference in %COP among the age groups. Similarly, no significant difference was found in %COP with gender, weight, height or BMI. In addition, we identified a normal reference range of %COP of 0.1-3.8%. In conclusion, in addition to the identification of steady levels of COP cells with age, we also identified a normal reference range of %COP, which could be used in future studies looking at musculoskeletal diseases in older populations.
循环骨祖细胞（COP）是一种血源性细胞，表达多种成骨细胞标志物，且能够在体内形成骨结节。虽然高比例的COP细胞（%COP）与血管钙化有关，但低%COP与残疾和虚弱有关。然而，年龄和性别匹配人群中%COP的参考范围以及%COP随年龄的变化情况仍不清楚。在悉尼西部对144名健康志愿者（年龄20 - 90岁，每十年10名男性和10名女性受试者）进行了一项横断面研究。通过流式细胞术对%COP进行定量分析。发现评分者间和评分者内具有较高的可靠性。在这个健康人群中，%COP的平均值为0.42。各年龄组之间的%COP没有显著差异。同样，在%COP与性别、体重、身高或体重指数之间也没有发现显著差异。此外，我们确定了%COP的正常参考范围为0.1 - 3.8%。总之，除了确定COP细胞随年龄的稳定水平外，我们还确定了%COP的正常参考范围，这可用于未来针对老年人群肌肉骨骼疾病的研究。
