Molecular Analysis of HLA-G in Women with High-Risk Pregnancy and Their Partners with Regard to Possible Complications.

The understanding of the molecular and biochemical characteristics of the human leukocyte antigen-G () is important because of the diverse influence of this antigen's polymorphisms on the course of a pregnancy. The aim of our study was to assess how the variation of the allele and the 14-bp ins/del polymorphism influence predisposition to a complicated pregnancy. The clinical material consisted of parental pairs with complicated pregnancies (210 women; 190 men). The control group included parental pairs without complications during pregnancy (89 women; 86 men). The study involved isolation of genome DNA from peripheral blood leukocytes, sequencing, and analysis of the 14-bp ins/del polymorphism in the 3'-untranslated region (3'-UTR) of the gene based on polymerase chain reaction (PCR). The most common allele in the group of women with complicated pregnancies was the allele. There were no statistically significant differences in the frequencies of the 14-bp ins/del polymorphism in the 3'UTR of the gene between the groups. Our results suggest that the risk of complications in pregnancy is influenced by the , , and alleles and is not influenced by the 14-bp ins/del polymorphism in the 3'UTR of the gene.