Ma Qi-Jun, Sun Mei-Hong, Lu Jing, Liu Ya-Jing, Hu Da-Gang, Hao Yu-Jin
National Key Laboratory of Crop Biology, National Research Center for Apple Engineering and Technology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An, Shandong 271018, China.
National Key Laboratory of Crop Biology, National Research Center for Apple Engineering and Technology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An, Shandong 271018, China
Plant Physiol. 2017 Aug;174(4):2348-2362. doi: 10.1104/pp.17.00502. Epub 2017 Jun 9.
Sugars play important roles in plant growth and development, crop yield and quality, as well as responses to abiotic stresses. Abscisic acid (ABA) is a multifunctional hormone. However, the exact mechanism by which ABA regulates sugar accumulation is largely unknown in plants. Here, we tested the expression profile of several sugar transporter and amylase genes in response to ABA treatment. and were isolated and genetically transformed into apple () to investigate their roles in ABA-induced sugar accumulation. The MdAREB2 transcription factor was found to bind to the promoters of the sugar transporter and amylase genes and activate their expression. Both MdAREB2 and MdSUT2 transgenic plants produced more soluble sugars than controls. Furthermore, MdAREB2 promoted the accumulation of sucrose and soluble sugars in an -dependent manner. Our results demonstrate that the ABA-responsive transcription factor MdAREB2 directly activates the expression of amylase and sugar transporter genes to promote soluble sugar accumulation, suggesting a mechanism by which ABA regulates sugar accumulation in plants.
糖类在植物生长发育、作物产量和品质以及对非生物胁迫的响应中发挥着重要作用。脱落酸（ABA）是一种多功能激素。然而，ABA调节糖积累的确切机制在植物中很大程度上尚不清楚。在这里，我们检测了几种糖转运蛋白和淀粉酶基因响应ABA处理的表达谱。并分离出[具体基因名称1]和[具体基因名称2]，并将其遗传转化到苹果（[苹果品种名称]）中，以研究它们在ABA诱导的糖积累中的作用。发现MdAREB2转录因子与糖转运蛋白和淀粉酶基因的启动子结合并激活它们的表达。MdAREB2和MdSUT2转基因植物都比对照产生了更多的可溶性糖。此外，MdAREB2以依赖[具体物质名称]的方式促进蔗糖和可溶性糖的积累。我们的结果表明，ABA响应转录因子MdAREB2直接激活淀粉酶和糖转运蛋白基因的表达，以促进可溶性糖积累，这提示了一种ABA调节植物糖积累的机制。
