Department of Ophthalmology, Tokyo Medical University, Ibaraki Medical Center, Ami, Japan.
Department of Ophthalmology, Tokyo Medical University, Tokyo, Japan.
Sci Rep. 2017 Jun 9;7(1):3150. doi: 10.1038/s41598-017-03529-8.
The purpose of the present study was to evaluate the intraretinal migration of the retinal pigment epithelium (RPE) cells in age-related macular degeneration (AMD) using polarimetry. We evaluated 155 eyes at various AMD stages. Depolarized light images were computed using a polarization-sensitive scanning laser ophthalmoscope (PS-SLO), and the degree of polarization uniformity was calculated using polarization-sensitive optical coherence tomography (OCT). Each polarimetry image was compared with the corresponding autofluorescence (AF) images at 488 nm (SW-AF) and at 787 nm (NIR-AF). Intraretinal RPE migration was defined by the presence of depolarization at intraretinal hyperreflective foci on PS-SLO and PS-OCT images, and by the presence of hyper-AF on both NIR-AF and SW-AF images. RPE migration was detected in 52 of 155 eyes (33.5%) and was observed in drusenoid pigment epithelial detachment (PED) and serous PED with significantly higher frequencies than in other groups (P = 0.015). The volume of the migrated RPE cluster in serous PED was significantly correlated with the volume of the PED (R = 0.26; P = 0.011). Overall, our results showed that intraretinal RPE migrations occurred in various AMD stages, and that they occurred more commonly in eyes with serous and drusenoid PED.
本研究旨在利用偏光镜评估年龄相关性黄斑变性（AMD）中的视网膜色素上皮（RPE）细胞的视网膜内迁移。我们评估了处于不同 AMD 阶段的 155 只眼睛。使用偏振敏感扫描激光检眼镜（PS-SLO）计算去偏振光图像，并使用偏振敏感光相干断层扫描（OCT）计算偏振均匀度。将每个偏光镜图像与相应的 488nm（SW-AF）和 787nm（NIR-AF）的自发荧光（AF）图像进行比较。在 PS-SLO 和 PS-OCT 图像上，视网膜内高反射性焦点存在去极化，并且在 NIR-AF 和 SW-AF 图像上均存在高 AF 时，定义为视网膜内 RPE 迁移。在 155 只眼中的 52 只（33.5％）中检测到 RPE 迁移，在玻璃膜疣样色素上皮脱离（PED）和浆液性 PED 中观察到的频率明显高于其他组（P=0.015）。浆液性 PED 中迁移的 RPE 簇的体积与 PED 的体积呈显著相关（R=0.26；P=0.011）。总体而言，我们的结果表明，视网膜内 RPE 迁移发生在各种 AMD 阶段，并且在具有浆液性和玻璃膜疣样 PED 的眼睛中更为常见。
