Kašpárková Věra, Humpolíček Petr, Capáková Zdenka, Bober Patrycja, Stejskal Jaroslav, Trchová Miroslava, Rejmontová Petra, Junkar Ita, Lehocký Marián, Mozetič Miran
Centre of Polymer Systems, Tomas Bata University in Zlin, 76001 Zlin, Czech Republic; Department of Fat, Surfactant, and Cosmetics Technology, Faculty of Technology, Tomas Bata University in Zlin, 760 01 Zlin, Czech Republic.
Centre of Polymer Systems, Tomas Bata University in Zlin, 76001 Zlin, Czech Republic; Polymer Centre, Faculty of Technology, Tomas Bata University in Zlin, 760 01 Zlin, Czech Republic.
Colloids Surf B Biointerfaces. 2017 Sep 1;157:309-316. doi: 10.1016/j.colsurfb.2017.05.066. Epub 2017 May 31.
Conducting polyaniline can be prepared and modified using several procedures, all of which can significantly influence its applicability in different fields of biomedicine or biotechnology. The modifications of surface properties are crucial with respect to the possible applications of this polymer in tissue engineering or as biosensors. Innovative technique for preparing polyaniline films via in-situ polymerization in colloidal dispersion mode using four stabilizers (poly-N-vinylpyrrolidone; sodium dodecylsulfate; Tween 20 and Pluronic F108) was developed. The surface energy, conductivity, spectroscopic features, and cell compatibility of thin polyaniline films were determined using contact-angle measurement, the van der Pauw method, Fourier-transform infrared spectroscopy, and assay conducted on mouse fibroblasts, respectively. The stabilizers significantly influenced not only the surface and electrical properties of the films but also their cell compatibility. Sodium dodecylsulfate seems preferentially to combine both the high conductivity and good cell compatibility. Moreover, the films with sodium dodecylsulfate were non-irritant for skin, which was confirmed by their in-vitro exposure to the 3D-reconstructed human tissue model.
导电聚苯胺可以通过多种方法制备和改性，所有这些方法都会显著影响其在生物医学或生物技术不同领域的适用性。表面性质的改性对于这种聚合物在组织工程或作为生物传感器的可能应用至关重要。开发了一种创新技术，通过在胶体分散模式下使用四种稳定剂（聚N-乙烯基吡咯烷酮、十二烷基硫酸钠、吐温20和普朗尼克F108）原位聚合来制备聚苯胺薄膜。分别使用接触角测量、范德堡方法、傅里叶变换红外光谱以及对小鼠成纤维细胞进行的测定，来确定聚苯胺薄膜的表面能、电导率、光谱特征和细胞相容性。稳定剂不仅显著影响薄膜的表面和电学性质，还影响其细胞相容性。十二烷基硫酸钠似乎优先兼具高电导率和良好的细胞相容性。此外，含有十二烷基硫酸钠的薄膜对皮肤无刺激性，这在其对三维重建的人体组织模型的体外暴露中得到了证实。
