Kucekova Zdenka, Humpolicek Petr, Kasparkova Vera, Perecko Tomas, Lehocký Marián, Hauerlandová Iva, Sáha Petr, Stejskal Jaroslav
Polymer Centre, Faculty of Technology, Tomas Bata University in Zlin, T.G.M. Sq. 5555, 760 01 Zlin, Czech Republic; Centre of Polymer Systems, Tomas Bata University in Zlin, T.G.M. Sq. 5555, 760 01 Zlin, Czech Republic.
Polymer Centre, Faculty of Technology, Tomas Bata University in Zlin, T.G.M. Sq. 5555, 760 01 Zlin, Czech Republic; Centre of Polymer Systems, Tomas Bata University in Zlin, T.G.M. Sq. 5555, 760 01 Zlin, Czech Republic.
Colloids Surf B Biointerfaces. 2014 Apr 1;116:411-7. doi: 10.1016/j.colsurfb.2014.01.027. Epub 2014 Jan 25.
Polyaniline colloids rank among promising application forms of this conducting polymer. Cytotoxicity, antibacterial activity, and neutrophil oxidative burst tests were performed on cells treated with colloidal polyaniline dispersions. The antibacterial effect of colloidal polyaniline against gram-positive and gram-negative bacteria was most pronounced for Bacillus cereus and Escherichia coli, with a minimum inhibitory concentration of 3,500 μg mL(-1). The data recorded on human keratinocyte (HaCaT) and a mouse embryonic fibroblast (NIH/3T3) cell lines using an MTT assay and flow cytometry indicated a concentration-dependent cytotoxicity of colloid, with the absence of cytotoxic effect at around 150 μg mL(-1). The neutrophil oxidative burst test then showed that colloidal polyaniline, in concentrations <150 μg mL(-1), was not able to stimulate the production of reactive oxygen species in neutrophils and whole human blood. However, it worked efficiently as a scavenger of those already formed.
聚苯胺胶体是这种导电聚合物很有前景的应用形式之一。对用胶体聚苯胺分散体处理的细胞进行了细胞毒性、抗菌活性和中性粒细胞氧化爆发试验。胶体聚苯胺对革兰氏阳性菌和革兰氏阴性菌的抗菌作用在蜡样芽孢杆菌和大肠杆菌中最为明显，最低抑菌浓度为3500μg mL(-1)。使用MTT法和流式细胞术在人角质形成细胞（HaCaT）和小鼠胚胎成纤维细胞（NIH/3T3）细胞系上记录的数据表明胶体具有浓度依赖性细胞毒性，在约150μg mL(-1)时无细胞毒性作用。中性粒细胞氧化爆发试验表明，浓度<150μg mL(-1)的胶体聚苯胺不能刺激中性粒细胞和全血中活性氧的产生。然而，它作为已形成的活性氧的清除剂却能有效发挥作用。
