Mahelová Leona, Slobodian Petr, Kocourková Karolína, Minařík Antonín, Moučka Robert, Trchová Miroslava, Martínková Martina, Skopalová Kateřina, Víchová Zdenka, Kašpárková Věra, Humpolíček Petr
Centre of Polymer Systems, University Institute, Tomas Bata University in Zlín, Trida T. Bati 5678, 76001, Zlin, Czech Republic.
Department of Physics and Materials Engineering, Faculty of Technology, Tomas Bata University in Zlin, Vavreckova 5669, 76001, Zlin, Czech Republic.
Heliyon. 2024 Mar 13;10(6):e27883. doi: 10.1016/j.heliyon.2024.e27883. eCollection 2024 Mar 30.
The coating of polymer substrate with polypyrrole, described herein with detailed know-how, represents a novel technique of surface functionalization. The choice of oxidizing agent and the polymerization time both affect the properties of the thin polypyrrole layer. The specific conductivity, free surface energy, thickness, topography, and FTIR spectra of polypyrrole layer were determined. The conductive coatings were further used to functionalize both isotropic and anisotropic electrospun polyurethane nanofibrous mats to show their applicability and study the bioactive effect of both the anisotropy and conductivity together. The morphology of composites was studied by means of atomic force microscopy and scanning electron microscopy. A complex cytocompatibility study was performed, including determining cytotoxicity by optical and fluorescence microscopy, the advanced qualification of cell morphology by cell-image analysis, and a study of stem cell behavior. The results clearly showed the significant impact of substrate modification on cells, especially on fibroblasts while the embryonic stem cells were less affected. This study shows not only the effective way to prepare a thin conducting layer based on polypyrrole but also demonstrates its importance for the fabrication of smart biomaterials.
本文详细介绍了用聚吡咯对聚合物基底进行涂层处理的方法，这是一种新型的表面功能化技术。氧化剂的选择和聚合时间都会影响聚吡咯薄层的性能。测定了聚吡咯层的比电导率、自由表面能、厚度、形貌和傅里叶变换红外光谱。导电涂层进一步用于对各向同性和各向异性的电纺聚氨酯纳米纤维毡进行功能化处理，以展示其适用性，并共同研究各向异性和导电性的生物活性效应。通过原子力显微镜和扫描电子显微镜研究了复合材料的形态。进行了一项复杂的细胞相容性研究，包括通过光学显微镜和荧光显微镜测定细胞毒性、通过细胞图像分析对细胞形态进行高级鉴定以及对干细胞行为的研究。结果清楚地表明了基底改性对细胞的显著影响，尤其是对成纤维细胞，而胚胎干细胞受到的影响较小。这项研究不仅展示了制备基于聚吡咯的薄导电层的有效方法，还证明了其在智能生物材料制造中的重要性。
