牛呼吸道疾病中支原体微生物的评估及其与临床症状的关系。
Evaluation of Mollicutes Microorganisms in Respiratory Disease of Cattle and Their Relationship to Clinical Signs.
作者信息
Tortorelli G, Carrillo Gaeta N, Mendonça Ribeiro B L, Miranda Marques L, Timenetsky J, Gregory L
机构信息
Department of Internal Medicine, School of Veterinary Medicine and Animal Science, University of São Paulo, Cidade Universitária, São Paulo, SP, Brazil.
Multidisciplinar Institute of Healthy, Federal University of Bahia, Vitoria da Conquista, Brazil.
出版信息
J Vet Intern Med. 2017 Jul;31(4):1215-1220. doi: 10.1111/jvim.14721. Epub 2017 Jun 10.
BACKGROUND
Bovine respiratory disease (BRD) is an important problem in cattle production that is responsible for economic losses in dairy herds. Mycoplasma spp. are described as an important etiological agent of BRD.
HYPOTHESIS
To evaluate the occurrence of the most important mycoplasmas in the lower respiratory tract of healthy and BRD cattle in relationship to clinical signs of BRD.
ANIMALS
Sixty young dairy cattle were classified as healthy (n = 32) or cattle showing clinical signs of BRD (n = 28).
METHODS
Tracheal lavage samples were collected and added to tubes containing Hayflick media. Mycoplasma spp. were identified by the presence of "fried egg" like colonies, biochemical tests and polymerase chain reaction (PCR). Occurrence of Mollicutes, M. bovis, M. mycoides subsp. mycoides SC and M. dispar was evaluated. The association between clinical signs of BRD and the presence of Mycoplasma spp. also was evaluated.
RESULTS
Colonies were obtained from a 1-year-old BRD calf only. However, species identification was not possible. Mollicutes (P = .035) and M. dispar (P = .036) were more common in BRD cattle. The relationship between Mollicutes and crackle (P = .057) was not significant. M. dispar was associated to tachypnea (P = .045) and mixed dyspnea (P = .003). Relationships to heart rate (P = .062) and crackle (P = .062) were not significant.
CONCLUSIONS AND CLINICAL IMPORTANCE
The results confirmed the importance of mycoplasma as an etiologic agent of BRD and suggested M. dispar as part of the respiratory microbiota and its possible role in the development of BRD.
背景
牛呼吸道疾病（BRD）是养牛生产中的一个重要问题，会给奶牛群造成经济损失。支原体属被认为是BRD的一种重要病原体。
假设
评估健康牛和患BRD的牛的下呼吸道中最重要的支原体的发生情况与BRD临床症状之间的关系。
动物
60头年轻奶牛被分为健康组（n = 32）或表现出BRD临床症状的牛组（n = 28）。
方法
采集气管冲洗样本并添加到含有海弗利克培养基的试管中。通过出现“煎蛋”样菌落、生化试验和聚合酶链反应（PCR）鉴定支原体属。评估柔膜菌纲、牛支原体、丝状支原体丝状亚种SC和差异支原体的发生情况。还评估了BRD临床症状与支原体属存在之间的关联。
结果
仅从一头1岁患BRD的小牛身上获得了菌落。然而，无法进行物种鉴定。柔膜菌纲（P = .035）和差异支原体（P = .036）在患BRD的牛中更常见。柔膜菌纲与啰音之间的关系（P = .057）不显著。差异支原体与呼吸急促（P = .045）和混合性呼吸困难（P = .003）相关。与心率（P = .062）和啰音（P = .062）的关系不显著。
结论及临床意义
结果证实了支原体作为BRD病原体的重要性，并表明差异支原体是呼吸道微生物群的一部分及其在BRD发生中的可能作用。
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