编码大肠杆菌K12 IMP脱氢酶的guaB基因座的核苷酸序列。
Nucleotide sequence of the guaB locus encoding IMP dehydrogenase of Escherichia coli K12.
作者信息
Tiedeman A A, Smith J M
出版信息
Nucleic Acids Res. 1985 Feb 25;13(4):1303-16. doi: 10.1093/nar/13.4.1303.
Abstract
IMP dehydrogenase, the product of the guaB locus in Escherichia coli K12, catalyzes the synthesis of XMP by the NAD+ dependent oxidation of IMP. The guaB locus has been subcloned from the Clarke and Carbon plasmid pLC34-10. The sequence of the guaB structural gene and surrounding DNA was determined by the dideoxy chain termination method of Sanger. The 1.533 kb guaB gene encodes an IMP dehydrogenase subunit of molecular weight 54,512. S1 nuclease mapping placed the site of guaBA mRNA initiation approximately 188 bp from the start of the guaB structural gene. The -10 and -35 regions that define the guaBA promoter were located upstream of the start of the guaBA transcription initiation site. The control region of approximately 188 bp does not show any obvious potential for secondary structure. A secondary lambda att site has been identified 42 bp distal to the guaB start codon.
摘要
IMP脱氢酶是大肠杆菌K12中guaB基因座的产物，它通过依赖NAD +的IMP氧化作用催化XMP的合成。guaB基因座已从克拉克和卡尔文质粒pLC34 - 10中进行亚克隆。guaB结构基因及周围DNA的序列通过桑格的双脱氧链终止法测定。1.533 kb的guaB基因编码一个分子量为54,512的IMP脱氢酶亚基。S1核酸酶图谱分析表明，guaBA mRNA起始位点距guaB结构基因起始处约188 bp。界定guaBA启动子的 - 10和 - 35区域位于guaBA转录起始位点起始处的上游。约188 bp的控制区域未显示出任何明显的二级结构潜力。在guaB起始密码子下游42 bp处鉴定出一个二级λ附着位点。
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