Laboratory of Biochemistry and biomedical materials, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
Laboratory of Biochemistry and biomedical materials, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
Carbohydr Polym. 2017 Sep 15;172:255-264. doi: 10.1016/j.carbpol.2017.05.035. Epub 2017 May 11.
A novel biodegradable chitin hernia patch was prepared by acetylation of chitosan fabric in our study. Physicochemical properties, cell compatibility and biodegradability of the chitin patch were quantified. Histopathological study of the functional experiment showed that this newly designed hernia patch promoted collagen deposition and neovascularization by significantly promoting the secretion of FGF1 and TGF-β1 in the early postoperative (P<0.01). Chitin patch caused less inflammation by inhibiting excessive expression of IL-6 and TNF-α when compared to the polypropylene mesh (P<0.01). Acceptable fibrosis was consistent with the results of immunohistochemistry studies. The density of FGF1 and TGF-β1 positive cells in the chitin patch group at 7 d was reduced to a lower level at 15 d (P<0.01). With regeneration of the defect abdominal wall, chitin patch degraded gradually, avoiding foreign body response and chronic complications. Our studies demonstrated that the newly designed chitin patch showed good promise for the hernia treatment.
在本研究中，通过壳聚糖织物的乙酰化制备了一种新型可生物降解的壳聚糖疝修补片。对壳聚糖补片的物理化学性质、细胞相容性和生物降解性进行了定量分析。功能实验的组织病理学研究表明，这种新设计的疝修补片通过显著促进 FGF1 和 TGF-β1 的分泌，在术后早期（P<0.01）促进胶原蛋白沉积和新生血管形成。与聚丙烯网片相比，壳聚糖补片通过抑制 IL-6 和 TNF-α 的过度表达，引起的炎症反应较少（P<0.01）。可接受的纤维化与免疫组织化学研究的结果一致。壳聚糖补片组在 7 d 时 FGF1 和 TGF-β1 阳性细胞的密度在 15 d 时降低到较低水平（P<0.01）。随着腹壁缺陷的再生，壳聚糖补片逐渐降解，避免了异物反应和慢性并发症。我们的研究表明，新设计的壳聚糖补片在疝治疗方面有很好的应用前景。
