School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China.
State Key Laboratory of Microbial Metabolism and School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Bioresour Technol. 2017 Dec;245(Pt B):1461-1468. doi: 10.1016/j.biortech.2017.05.191. Epub 2017 Jun 1.
The aim of this work was to study the effects of deleting acetate transporter gene ADY2 on growth and fermentation of Saccharomyces cerevisiae in the presence of inhibitors. Comparative transcriptome analysis revealed that three genes encoding plasma membrane carboxylic acid transporters, especially ADY2, were significantly downregulated under the zinc sulfate addition condition in the presence of acetic acid stress, and the deletion of ADY2 improved growth of S. cerevisiae under acetic acid, ethanol and hydrogen peroxide stresses. Consistently, a concomitant increase in ethanol production by 14.7% in the presence of 3.6g/L acetic acid was observed in the ADY2 deletion mutant of S. cerevisiae BY4741. Decreased intracellular acetic acid, ROS accumulation, and plasma membrane permeability were observed in the ADY2 deletion mutant. These findings would be useful for developing robust yeast strains for efficient ethanol production.
本工作旨在研究在抑制剂存在的情况下，删除醋酸盐转运基因 ADY2 对酿酒酵母生长和发酵的影响。比较转录组分析表明，在添加硫酸锌的条件下，三株编码质膜羧酸转运蛋白的基因，特别是 ADY2，在存在醋酸胁迫时显著下调，而 ADY2 的缺失提高了酿酒酵母在醋酸、乙醇和过氧化氢胁迫下的生长。一致地，在 3.6g/L 醋酸存在的情况下，观察到酿酒酵母 BY4741 的 ADY2 缺失突变体的乙醇产量增加了 14.7%。在 ADY2 缺失突变体中观察到细胞内醋酸、ROS 积累和质膜通透性降低。这些发现将有助于开发高效乙醇生产的稳健酵母菌株。
