Université de Versailles-Saint-Quentin, Montigny-le-Bretonneux, France.
Université Paris Descartes, Sorbonne Paris Cité, Laboratoire d'Excellence Inflamex, Paris, France.
Ann Rheum Dis. 2017 Sep;76(9):1614-1622. doi: 10.1136/annrheumdis-2016-211064. Epub 2017 Jun 12.
Altered microbiota composition or dysbiosis is suspected to be implicated in the pathogenesis of chronic inflammatory diseases, such as spondyloarthritis (SpA) and rheumatoid arthritis (RA).
16S ribosomal RNA gene sequencing was performed on faecal DNA isolated from stool samples in two consecutive cross-sectional cohorts, each comprising three groups of adult volunteers: SpA, RA and healthy controls (HCs). In the second study, HCs comprised a majority of aged-matched siblings of patients with known HLA-B27 status. Alpha and beta diversities were assessed using QIIME, and comparisons were performed using linear discriminant analysis effect size to examine differences between groups.
In both cohorts, dysbiosis was evidenced in SpA and RA, as compared with HCs, and was disease specific. A restriction of microbiota biodiversity was detected in both disease groups. The most striking change was a twofold to threefold increased abundance of in SpA, as compared with both RA and HCs that was significant in both studies and positively correlated with disease activity in patients having a history of inflammatory bowel disease (IBD). Among HCs, significant difference in microbiota composition were also detected between HLA-B27+ and HLA-B27 negative siblings, suggesting that genetic background may influence gut microbiota composition.
Our results suggest that distinctive dysbiosis characterise both SpA and RA and evidence a reproducible increase in that appears specific for SpA and a marker of disease activity. This observation is consistent with the known proinflammatory role of this bacteria and its association with IBD. It may provide an explanation for the link that exists between SpA and IBD.
改变的微生物群落组成或失调被怀疑与慢性炎症性疾病的发病机制有关，如脊柱关节炎（SpA）和类风湿关节炎（RA）。
对来自两个连续横断面队列的粪便样本中分离的粪便 DNA 进行 16S 核糖体 RNA 基因测序，每个队列均包含三组成年志愿者：SpA、RA 和健康对照（HCs）。在第二项研究中，HCs 主要是已知 HLA-B27 状态的患者的年龄匹配的兄弟姐妹。使用 QIIME 评估α和β多样性，并使用线性判别分析效应大小进行比较，以检查组间差异。
在两个队列中，与 HCs 相比，SpA 和 RA 中均存在菌群失调，且具有疾病特异性。在两个疾病组中均检测到微生物群落生物多样性受限。最显著的变化是与 RA 和 HCs 相比，SpA 中增加了两倍至三倍，在两项研究中均具有统计学意义，并且与患有炎症性肠病（IBD）病史的患者的疾病活动度呈正相关。在 HCs 中，还检测到 HLA-B27+和 HLA-B27 阴性兄弟姐妹之间的微生物群落组成存在显著差异，这表明遗传背景可能影响肠道微生物群落组成。
我们的研究结果表明，SpA 和 RA 都存在独特的菌群失调，并且存在可重复的增加，这似乎是 SpA 的特异性标志物，也是疾病活动度的标志物。这一观察结果与该细菌的已知促炎作用及其与 IBD 的关联一致。它可能为 SpA 和 IBD 之间存在的联系提供了解释。
