采用免疫组织化学法检测外泌体标志物（CD63和CD9）在切除的恶性和非恶性胰腺标本中的表达模式。
Exosomal Markers (CD63 and CD9) Expression Pattern Using Immunohistochemistry in Resected Malignant and Nonmalignant Pancreatic Specimens.
作者信息
Khushman Moh'd, Bhardwaj Arun, Patel Girijesh Kumar, Laurini Javier Ariel, Roveda Kelly, Tan Marcus C, Patton Mary C, Singh Seema, Taylor William, Singh Ajay P
机构信息
From the *Medical Oncology and †Department of Oncologic Sciences, Mitchell Cancer Institute, and Departments of ‡Pathology and §Surgical Oncology, The University of South Alabama, Mobile, AL.
出版信息
Pancreas. 2017 Jul;46(6):782-788. doi: 10.1097/MPA.0000000000000847.
OBJECTIVES
Exosomes are important mediators in intercellular communications and play a role in cancer progression and metastasis. Exosomal membranes are enriched in endosome-specific tetraspanins (CD9 and CD63). Here, we explored the expression of CD63 and CD9 utilizing immunohistochemistry in malignant and nonmalignant cells in 29 resected pancreatic specimens (RPSs) of mixed racial background.
METHODS
The pathologic tissues (PTs) and adjacent normal tissues (ANTs) in each RPS were stained for CD63 and CD9. Two pathologists independently scored the expression of CD63 and CD9. Staining intensity was graded from 1 to 3. Staining percentage was estimated in 10% increments. An average Quick score (Q score) (intensity × percentage of staining) was calculated. Unpaired t test was used for statistical analysis.
RESULTS
The mean multiplicative Q score for CD63 and CD9 expression is higher in PTs (209 and 72) compared with ANTs (154 and 24) (P = 0.0041, P = 0.0018), respectively. The Mean Q score for CD63 and CD9 expression is higher in the malignant PTs (231 and 85) compared with ANTs (129 and 25) (P < 0.0001 and P < 0.0124).
CONCLUSIONS
Exosomal markers (CD63 and CD9) expression assessment using immunohistochemistry is feasible in RPS. The expression of CD63 and CD9 is higher in PTs and malignant PTs compared with their ANTs.
目的
外泌体是细胞间通讯的重要介质，在癌症进展和转移中发挥作用。外泌体膜富含内体特异性四跨膜蛋白（CD9和CD63）。在此，我们利用免疫组织化学方法，在29例混合种族背景的切除胰腺标本（RPS）的恶性和非恶性细胞中，探究了CD63和CD9的表达情况。
方法
对每个RPS中的病理组织（PT）和相邻正常组织（ANT）进行CD63和CD9染色。两名病理学家独立对CD63和CD9的表达进行评分。染色强度分为1至3级。染色百分比以10%的增量进行估计。计算平均快速评分（Q评分）（强度×染色百分比）。采用非配对t检验进行统计分析。
结果
与ANT（分别为154和24）相比，PT中CD63和CD9表达的平均乘积Q评分更高（分别为209和72）（P = 0.0041，P = 0.0018）。与ANT（分别为129和25）相比，恶性PT中CD63和CD9表达的平均Q评分更高（分别为231和85）（P < 0.0001和P < 0.0124）。
结论
在RPS中，使用免疫组织化学评估外泌体标志物（CD63和CD9）的表达是可行的。与ANT相比，PT和恶性PT中CD63和CD9的表达更高。
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