Elson C O, Machelski E, Weiserbs D B
Gastroenterology. 1985 Aug;89(2):321-7. doi: 10.1016/0016-5085(85)90332-4.
Many patients with Crohn's disease have suppressor T cells circulating in the peripheral blood that are potent inhibitors of immunoglobulin synthesis in vitro. The purpose of this study was to examine the regulatory effects of T cells isolated from the lamina propria of patients with Crohn's disease or of patients with other diseases. Lamina propria cells were isolated from surgically resected intestine by sequential ethylenediaminetetraacetic acid and collagenase incubations. T cells were purified from lamina propria either by anti-F(ab)2' affinity columns or by a panning technique using monoclonal anti-T cell antibodies. Changes in the pokeweed mitogen-stimulated synthesis of immunoglobulin M, immunoglobulin G, and immunoglobulin A by normal peripheral blood indicator lymphocytes, as measured by sandwich enzyme-linked immunosorbent assay, was used as an index of help and suppression. Helper T-cell activity was sought by coculturing lamina propria T cells with normal peripheral blood cells plus pokeweed mitogen. Helper T-cell activity was found among lamina propria T-cell populations of patients and controls to a roughly comparable extent; help was provided for all isotypes. Suppressor T-cell activity was sought by coculturing lamina propria T-cells with normal B cells plus irradiated normal T cells plus pokeweed mitogen. No significant suppressor T-cell activity was observed in these cocultures, whether the cells were obtained from control intestine, the grossly uninvolved margin of Crohn's disease intestine, or actively inflamed Crohn's disease intestine. We conclude that suppressor T-cell activity of the sort found previously in the peripheral blood of patients with Crohn's disease is not demonstrable in the intestinal lesions of Crohn's disease. Helper T-cell activity is the predominant regulatory activity in both control and inflamed intestinal lamina propria.
许多克罗恩病患者的外周血中循环存在抑制性T细胞，这些细胞在体外是免疫球蛋白合成的强效抑制剂。本研究的目的是检测从克罗恩病患者或其他疾病患者的固有层分离出的T细胞的调节作用。通过依次用乙二胺四乙酸和胶原酶孵育，从手术切除的肠道中分离固有层细胞。通过抗F(ab)2'亲和柱或使用单克隆抗T细胞抗体的淘选技术从固有层中纯化T细胞。用夹心酶联免疫吸附测定法测量正常外周血指示淋巴细胞受商陆有丝分裂原刺激后免疫球蛋白M、免疫球蛋白G和免疫球蛋白A合成的变化，以此作为辅助和抑制的指标。通过将固有层T细胞与正常外周血细胞加商陆有丝分裂原共培养来寻找辅助性T细胞活性。在患者和对照的固有层T细胞群体中发现辅助性T细胞活性的程度大致相当；对所有同种型都有辅助作用。通过将固有层T细胞与正常B细胞加经辐照的正常T细胞加商陆有丝分裂原共培养来寻找抑制性T细胞活性。在这些共培养物中未观察到明显的抑制性T细胞活性，无论细胞是从对照肠道、克罗恩病肠道大体未受累边缘还是活动性炎症的克罗恩病肠道获得的。我们得出结论，先前在克罗恩病患者外周血中发现的那种抑制性T细胞活性在克罗恩病的肠道病变中无法证实。辅助性T细胞活性是对照和炎症性肠道固有层中的主要调节活性。
