Verspaget H W, Peña A S, Weterman I T, Lamers C B
Department of Gastroenterology and Hepatology, University Hospital, Leiden, The Netherlands.
Gut. 1988 Apr;29(4):503-10. doi: 10.1136/gut.29.4.503.
In vitro immunoglobulin synthesis by isolated intestinal mononuclear cells of macroscopically normal mucosa from patients with Crohn's disease has been studied and results compared with those obtained with cells from normal mucosa of resection specimens from patients with colonic carcinoma, or other intestinal disorders. The total lamina propria mononuclear cells (LPMC) in Crohn's disease produced spontaneously less IgA and more IgG than the control groups, but no difference was observed for IgM. An enriched lymphocyte fraction (LPL) of the Crohn's disease patients showed a higher spontaneous synthesis of IgA and IgG when compared with controls. In contrast, another fraction enriched for macrophages (LPM) produced spontaneously less IgA, IgG, and IgM in Crohn's, than in control patients. Incubation with pokeweed mitogen (PWM) decreased immunoglobulin synthesis by LPMC in Crohn's disease and controls, and this was enhanced by simultaneous incubation with concanavalin A. In controls PWM stimulation increased immunoglobulin synthesis by LPL and decreased immunoglobulin synthesis by LPM. In Crohn's disease, however, PWM had no effect on either fraction. This study shows major differences in the regulation of the immunoglobulin synthesis by intestinal cells between Crohn's disease and controls.
对克罗恩病患者宏观正常黏膜分离出的肠道单核细胞进行体外免疫球蛋白合成研究，并将结果与结肠癌患者或其他肠道疾病患者切除标本正常黏膜细胞的研究结果进行比较。克罗恩病固有层单核细胞（LPMC）总体自发产生的IgA比对照组少，IgG比对照组多，但IgM未观察到差异。与对照组相比，克罗恩病患者富集淋巴细胞组分（LPL）显示出更高的IgA和IgG自发合成。相反，另一个富含巨噬细胞的组分（LPM）在克罗恩病患者中自发产生的IgA、IgG和IgM比对照患者少。用商陆有丝分裂原（PWM）孵育可降低克罗恩病和对照组中LPMC的免疫球蛋白合成，同时与伴刀豆球蛋白A孵育可增强这种降低作用。在对照组中，PWM刺激可增加LPL的免疫球蛋白合成并降低LPM的免疫球蛋白合成。然而，在克罗恩病中，PWM对这两个组分均无影响。这项研究表明，克罗恩病与对照组之间肠道细胞免疫球蛋白合成的调节存在重大差异。
