Pallone F, Fais S, Squarcia O, Biancone L, Pozzilli P, Boirivant M
Gut. 1987 Jun;28(6):745-53. doi: 10.1136/gut.28.6.745.
In the present study the state of activation of either peripheral blood and intestinal lamina propria mononuclear cells in Crohn's disease was defined by investigating the expression of early activation antigens (namely the 4F2 antigen, the transferrin receptor and the interleukin-2 receptor). The expression of 4F2 and T9 antigens was greatly increased--in the peripheral blood and in the intestinal lamina propria whereas the proportion of interleukin-2 receptor bearing cells was much less pronounced. The counts of early activation antigens bearing cells in the lamina propria were quite comparable with those of the autologous peripheral cells. In the peripheral blood counts of 4F2 and T9 positive cells were very high in patients with active Crohn's disease but patients with quiescent disease also had a significantly raised proportion of 4F2 and T9 bearing cells. Only in those patients with no evidence of macroscopic disease (namely those resected without recurrence) the counts of early activation antigens bearing cells were within the normal range. The in vitro mitogen induced expression of early activation antigens on either peripheral and intestinal mononuclear cells of patients with Crohn's disease proved to be both quantitatively and qualitatively similar to that of the controls showing the full expression of 4F2, transferrin receptor, and interleukin-2 receptor. While demonstrating that in Crohn's disease there was no intrinsic defect of generation and expression of growth factors receptors by peripheral and intestinal lymphocytes, these results showed that there was a divergence in the expression of early activation antigens in vivo and in vitro. This would indicate that in Crohn's disease there is an in vivo increased population of preactivated rather than fully activated lymphocytes consisting of 4F2 and T9 bearing cells. The high proportion of these cells in the peripheral blood and in the intestine suggests that a chronic immune activation is present in these patients outside as well as within the affected bowel.
在本研究中，通过调查早期激活抗原（即4F2抗原、转铁蛋白受体和白细胞介素-2受体）的表达情况，确定了克罗恩病患者外周血和肠道固有层单核细胞的激活状态。4F2和T9抗原的表达在外周血和肠道固有层中显著增加，而白细胞介素-2受体阳性细胞的比例变化则不太明显。固有层中携带早期激活抗原的细胞计数与自体外周细胞的计数相当。在活动期克罗恩病患者的外周血中，4F2和T9阳性细胞的计数非常高，但病情静止期的患者中，携带4F2和T9的细胞比例也显著升高。只有那些没有明显宏观病变证据的患者（即切除后未复发的患者），携带早期激活抗原的细胞计数才在正常范围内。克罗恩病患者外周血和肠道单核细胞上，体外有丝分裂原诱导的早期激活抗原表达，在数量和质量上都与对照组相似，显示出4F2、转铁蛋白受体和白细胞介素-2受体的充分表达。这些结果表明，克罗恩病患者外周血和肠道淋巴细胞产生和表达生长因子受体不存在内在缺陷，但体内和体外早期激活抗原的表达存在差异。这表明在克罗恩病中，体内预激活而非完全激活的淋巴细胞（由携带4F2和T9的细胞组成）数量增加。这些细胞在外周血和肠道中的高比例表明，这些患者在患病肠道内外都存在慢性免疫激活。
