Van Gossum A, Dupont E, Schandene L, Cremer M, Wybran J
Department of Gastroenterology, Hôpital Erasme, Free University of Brussels, Belgium.
Dig Dis Sci. 1988 Aug;33(8):945-50. doi: 10.1007/BF01535989.
Immune-mediated mechanisms and genetic factors are believed to be involved in the pathogenesis of Crohn's disease. We studied T- and B-cell subpopulation proportions and various functional assays, including proliferative responses to PHA and Con A, Con A-induced suppressive activity, and natural killer cell assay toward the K562 cell line, in the peripheral blood of 22 patients with inactive familial Crohn's disease and their 35 healthy relatives including nine families. HLA-A, -B, and -DR antigens were determined in all the subjects. With the exception of minor abnormalities of suppressor cell activity present in some relatives of two families, neither significant impairments of immunological parameters in patients or their relatives nor concordant segregation of HLA haplotypes and disease were observed. These data indicate that peripheral immune abnormalities previously described in patients with Crohn's disease do not constitute primary factors involved in the disease itself and that familial incidence in Crohn's disease cannot be linked to immunological markers presently studied.
免疫介导机制和遗传因素被认为与克罗恩病的发病机制有关。我们研究了22例非活动期家族性克罗恩病患者及其35名健康亲属（包括9个家庭）外周血中的T细胞和B细胞亚群比例以及各种功能检测，包括对PHA和Con A的增殖反应、Con A诱导的抑制活性以及对K562细胞系的自然杀伤细胞检测。对所有受试者进行了HLA - A、- B和 - DR抗原检测。除了两个家族的一些亲属存在轻微的抑制细胞活性异常外，未观察到患者或其亲属的免疫参数有明显损害，也未观察到HLA单倍型与疾病的一致分离。这些数据表明，先前在克罗恩病患者中描述的外周免疫异常并非该疾病本身的主要因素，并且克罗恩病的家族发病率与目前研究的免疫标志物无关。
