Parmar Nidhi R, Pandit Prabhakar D, Purohit Hemant J, Nirmal Kumar J I, Joshi Chaitanya G
Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, 388001 Gujarat India.
Environmental Genomics Division, CSIR-National Environmental Engineering Research Institute, Nagpur, Maharashtra India.
Indian J Microbiol. 2017 Jun;57(2):226-234. doi: 10.1007/s12088-016-0635-z. Epub 2017 Jan 16.
Comparative metagenomics approach has been used in this study to discriminate colonization of methanogenic population in different breeds of cattle. We compared two Indian cattle breeds (Gir and Kankrej) and two exotic cattle (Holstein and Jersey) breeds. Using a defined dietary plan for selected Indian varieties, the diet dependent shifts in microbial community and abundance of the enzymes associated with methanogenesis were studied. This data has been compared with the available rumen metagenome data from Holstein and Jersey dairy cattle. The abundance of genes for methanogenesis in Holstein and Jersey cattle came from order whereas, majority of the enzymes for methanogenesis in Gir and Kankrej cattle came from order. The study suggested that by using slow/less digestible feed, the propionate levels could be controlled in rumen; and in turn, this would also help in further reducing the hydrogenotrophic production of methane. The study proposes that with the designed diet plan the overall methanogenic microbial pool or the individual methanogens could be targeted for development of methane mitigation strategies.
本研究采用比较宏基因组学方法来区分不同品种牛中产甲烷菌群的定殖情况。我们比较了两个印度牛品种（吉尔牛和坎克雷吉牛）以及两个外来牛品种（荷斯坦牛和泽西牛）。通过为选定的印度品种制定特定的饮食计划，研究了饮食依赖型微生物群落的变化以及与甲烷生成相关的酶的丰度。该数据已与来自荷斯坦奶牛和泽西奶牛的现有瘤胃宏基因组数据进行了比较。荷斯坦牛和泽西牛中甲烷生成基因的丰度来自[具体菌目]，而吉尔牛和坎克雷吉牛中大部分甲烷生成酶来自[具体菌目]。该研究表明，通过使用消化缓慢/易消化性低的饲料，可以控制瘤胃中的丙酸水平；进而，这也将有助于进一步减少氢营养型甲烷的产生。该研究提出，通过设计的饮食计划，可针对整体产甲烷微生物库或单个产甲烷菌制定甲烷减排策略。
