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显示串联重复序列不同排列的寡核苷酸和ND-FISH以及小麦背景下簇毛麦染色体的鉴定

Oligonucleotides and ND-FISH Displaying Different Arrangements of Tandem Repeats and Identification of Dasypyrum villosum Chromosomes in Wheat Backgrounds.

作者信息

Xiao Zhiqiang, Tang Shuyao, Qiu Ling, Tang Zongxiang, Fu Shulan

机构信息

Provincial Key Laboratory of Plant Breeding and Genetics, Sichuan Agriculture University, Chengdu 611130, China.

Institute of Ecological Agriculture, Sichuan Agricultural University, Chengdu 611130, China.

出版信息

Molecules. 2017 Jun 14;22(6):973. doi: 10.3390/molecules22060973.

DOI:10.3390/molecules22060973
PMID:28613230
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6152725/
Abstract

Oligonucleotide probes and the non-denaturing fluorescence in situ hybridization (ND-FISH) technique are widely used to analyze plant chromosomes because they are convenient tools. New oligonucleotide probes, Oligo-Ku, Oligo-3B117.1, Oligo-3B117.2, Oligo-3B117.2.1, Oligo-3B117.3, Oligo-3B117.4, Oligo-3B117.5, Oligo-3B117.6, Oligo-pTa71A-1, Oligo-pTa71A-2, Oligo-pTa71B-1, Oligo-pTa71B-2, Oligo-pTa71C-1, Oligo-pTa71C-2, Oligo-pTa71C-3 and Oligo-pTa71D were designed based on the repetitive sequences KU.D15.15, pSc119.2-like sequence 3B117 and pTa71. Oligonucleotide probe (GT)₇ was also used. Oligo-Ku and (GT)₇ can be together used to identify from wheat chromosomes and to distinguish individual chromosomes. The oligonucleotide probes that were derived from the same repeat sequence displayed different signal intensity and hybridization sites on the same chromosomes. Both the length and the nucleotide composition of oligonucleotide probes determined their signal intensity. For example, Oligo-3B117.2 (25 bp) and Oligo-pTa71A-2 (46 bp) produced the strongest signals on chromosomes of wheat ( L.), rye ( L.), barley ( ssp. ) or , the signal of Oligo-3B117.4 (18 bp) on the short arm of 7B chromosome was weaker than that of Oligo-3B117.2.1 (15 bp) and Oligo-3B117.3 (16 bp), and Oligo-pTa71A-1 (38 bp) produced the same strong signals as Oligo-pTa71A-2 did on 1B and 6B chromosomes, but its signals on 1R and 1V chromosomes were weaker than the ones of Oligo-pTa71A-2. Oligonucleotide probes and ND-FISH analysis can reflect the distribution and structural statues of different segments of tandem repeats on chromosomes. The possible reasons why different segments derived from the same repeat sequence produced different signal patterns are discussed.

摘要

寡核苷酸探针和非变性荧光原位杂交（ND-FISH）技术因其便捷性而被广泛用于植物染色体分析。基于重复序列KU.D15.15、类pSc119.2序列3B117和pTa71设计了新的寡核苷酸探针，即Oligo-Ku、Oligo-3B117.1、Oligo-3B117.2、Oligo-3B117.2.1、Oligo-3B117.3、Oligo-3B117.4、Oligo-3B117.5、Oligo-3B117.6、Oligo-pTa71A-1、Oligo-pTa71A-2、Oligo-pTa71B-1、Oligo-pTa71B-2、Oligo-pTa71C-1、Oligo-pTa71C-2、Oligo-pTa71C-3和Oligo-pTa71D。还使用了寡核苷酸探针(GT)₇。Oligo-Ku和(GT)₇可共同用于从小麦染色体中进行识别并区分单个染色体。源自相同重复序列的寡核苷酸探针在同一染色体上显示出不同的信号强度和杂交位点。寡核苷酸探针的长度和核苷酸组成均决定了它们的信号强度。例如，Oligo-3B117.2（25 bp）和Oligo-pTa71A-2（46 bp）在小麦（L.）、黑麦（L.）、大麦（ssp.）或的染色体上产生最强信号，7B染色体短臂上Oligo-3B117.4（18 bp）的信号弱于Oligo-3B117.2.1（15 bp）和Oligo-3B117.3（16 bp），并且Oligo-pTa71A-1（38 bp）在1B和6B染色体上产生与Oligo-pTa71A-2相同的强信号，但它在1R和1V染色体上的信号弱于Oligo-pTa71A-2。寡核苷酸探针和ND-FISH分析可以反映串联重复不同区段在染色体上的分布和结构状态。文中讨论了源自相同重复序列的不同区段产生不同信号模式的可能原因。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf7c/6152725/8822feb2ff07/molecules-22-00973-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf7c/6152725/b274ef5c7cb8/molecules-22-00973-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf7c/6152725/cdc415f1b185/molecules-22-00973-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf7c/6152725/b76b45c30fc4/molecules-22-00973-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf7c/6152725/3945b5844aab/molecules-22-00973-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf7c/6152725/8822feb2ff07/molecules-22-00973-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf7c/6152725/b274ef5c7cb8/molecules-22-00973-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf7c/6152725/cdc415f1b185/molecules-22-00973-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf7c/6152725/b76b45c30fc4/molecules-22-00973-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf7c/6152725/3945b5844aab/molecules-22-00973-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf7c/6152725/8822feb2ff07/molecules-22-00973-g005.jpg

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