College of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, China.
Provincial Key Laboratory for Plant Genetics and Breeding, Wenjiang, Chengdu 611130, Sichuan, China.
Molecules. 2019 Mar 21;24(6):1126. doi: 10.3390/molecules24061126.
Non-denaturing fluorescence in situ hybridization (ND-FISH) has been used to distinguish wheat chromosomes and to detect alien chromosomes in the wheat genome. In this study, five different oligonucleotide probes were used with ND-FISH to examine 21 wheat cultivars and lines. These oligonucleotide probes distinguished 42 wheat chromosomes and also detected rye chromatin in the wheat genome. Moreover, the signal patterns of the oligonucleotide probes Oligo-pTa535-1 and Oligo-pSc119.2-1 showed high polymorphism in the wheat chromosomes. A total of 17.6% of the A group chromosomes, 25.9% of the B group chromosomes and 8.9% of the D group chromosomes showed obvious mutations when they were compared to the standard ND-FISH signal patterns, and most of them were Oligo-pSc119.2-1 mutants. The results suggested that these polymorphisms could be induced by the crossing of wheat cultivars. The results provided more information for the further application of oligonucleotide probes and ND-FISH.
非变性荧光原位杂交（ND-FISH）已被用于区分小麦染色体和检测小麦基因组中的异源染色体。本研究使用了 5 种不同的寡核苷酸探针进行 ND-FISH 分析，以检测 21 个小麦品种和系。这些寡核苷酸探针区分了 42 条小麦染色体，并在小麦基因组中检测到了黑麦染色质。此外，寡核苷酸探针 Oligo-pTa535-1 和 Oligo-pSc119.2-1 的信号模式在小麦染色体上表现出高度多态性。与标准 ND-FISH 信号模式相比，A 组染色体中有 17.6%、B 组染色体中有 25.9%和 D 组染色体中有 8.9%的染色体明显发生了突变，其中大多数是 Oligo-pSc119.2-1 突变体。这些结果表明，这些多态性可能是由小麦品种杂交引起的。这些结果为寡核苷酸探针和 ND-FISH 的进一步应用提供了更多信息。
