开发用于大麻（L.）染色体鉴定的寡核苷酸探针

Developing Oligo Probes for Chromosomes Identification in Hemp ( L.).

作者信息

Romanov Dmitry V, Karlov Gennady I, Divashuk Mikhail G

机构信息

All-Russia Research Institute of Agricultural Biotechnology, 127550 Moscow, Russia.

出版信息

Plants (Basel). 2022 Jul 22;11(15):1900. doi: 10.3390/plants11151900.

DOI:10.3390/plants11151900

PMID:35893604

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9332022/

Abstract

Hemp ( L., 2n = 20) is a valuable crop that is successfully used as a food, technical and medicinal crop. It is a dioecious plant with an XX\XY sex determination system. Some chromosomes of have almost the same lengths and centromeric indexes. Cytogenetic markers help to distinguish similar plant chromosomes, including sex chromosomes, which is important for the breeding process. Two repeats (CS-1 and CS-237) were used to develop labeled oligo-probes for rapid and low-cost oligo-FISH. These oligos can be recommended for use as cytological markers to distinguish sex chromosomes (X and Y) and somatic chromosome pairs 3, 6, and 8 by rapid oligo-FISH in a short time.

摘要

大麻（L.，2n = 20）是一种有价值的作物，已成功用作粮食作物、工业作物和药用作物。它是一种雌雄异株植物，具有XX\XY性别决定系统。其一些染色体的长度和着丝粒指数几乎相同。细胞遗传学标记有助于区分相似的植物染色体，包括性染色体，这对育种过程很重要。利用两个重复序列（CS-1和CS-237）开发了标记寡核苷酸探针，用于快速且低成本的寡核苷酸荧光原位杂交（oligo-FISH）。这些寡核苷酸可推荐用作细胞学标记，通过快速寡核苷酸荧光原位杂交在短时间内区分性染色体（X和Y）以及体细胞染色体对3、6和8。