Tsinghua University, Department of Automation, Beijing, China.
University of Connecticut, Biomedical Engineering, and Electrical and Computer Engineering, Storrs, Connecticut, United States.
J Biomed Opt. 2017 Jun 1;22(6):66006. doi: 10.1117/1.JBO.22.6.066006.
Fourier ptychographic microscopy (FPM) is a recently developed technique stitching low-resolution images in Fourier domain to realize wide-field high-resolution imaging. However, the time-consuming process of image acquisition greatly narrows its applications in dynamic imaging. We report a wavelength multiplexing strategy to speed up the acquisition process of FPM several folds. A proof-of-concept system is built to verify its feasibility. Distinguished from many current multiplexing methods in Fourier domain, we explore the potential of high-speed FPM in spectral domain. Compatible with most existing FPM methods, our strategy provides an approach to high-speed gigapixel microscopy. Several experimental results are also presented to validate the strategy.
傅里叶叠层显微镜（FPM）是一种新兴的技术，通过在傅里叶域中拼接低分辨率图像来实现宽场高分辨率成像。然而，图像采集过程耗时较长，极大地限制了其在动态成像中的应用。我们报告了一种波长复用策略，可以将 FPM 的采集速度提高数倍。构建了一个概念验证系统来验证其可行性。与当前傅里叶域中的许多复用方法不同，我们探索了高速 FPM 在光谱域中的潜力。与大多数现有的 FPM 方法兼容，我们的策略为高速千兆像素显微镜提供了一种方法。还呈现了一些实验结果来验证该策略。
