Hansson E, Isacsson H, Sellström A
Acta Physiol Scand. 1984 Aug;121(4):333-41. doi: 10.1111/j.1748-1716.1984.tb07464.x.
Primary astroglial cultures were grown from newborn rat cerebral hemispheres. Cultures, aged 7 and 14 days, were analysed with respect to their capacity to accumulate radioactive GABA and dopamine. Concentrative high-affinity uptake, showing Na+-dependence was observed for GABA where Km and Vmax were not significantly altered with age of the culture. No Na+-dependent high-affinity transport of dopamine was observed. The addition of 0.1 mM dibutyryl-cyclic-3'-5'-adenosinemonophosphate (dB-cAMP) after removal of fetal calf-serum reduced the Vmax as well as the Km of 3H-GABA uptake. The ionic dependence of 3H-GABA and 3H-dopamine was not significantly altered following this treatment. The results are difficult to interpret in terms of a potentiated differentiation of the cells.
原代星形胶质细胞培养物取自新生大鼠的大脑半球。对培养7天和14天的培养物进行分析，以研究它们积累放射性γ-氨基丁酸（GABA）和多巴胺的能力。观察到GABA存在依赖于钠离子的高亲和力浓缩摄取，其米氏常数（Km）和最大反应速度（Vmax）不会随培养物的年龄而显著改变。未观察到多巴胺的依赖于钠离子的高亲和力转运。去除胎牛血清后添加0.1 mM二丁酰环磷腺苷（dB-cAMP）会降低3H-GABA摄取的Vmax以及Km。此处理后，3H-GABA和3H-多巴胺的离子依赖性未显著改变。就细胞的增强分化而言，这些结果难以解释。
