Khan M A Hannan, Shareef P A A, Rehman Abdur, Ullah Rizwan, Rehman Lubna, Abidi S M A
Section of Parasitology, Department of Zoology, Aligarh Muslim University, Aligarh, India.
J Parasit Dis. 2017 Jun;41(2):423-428. doi: 10.1007/s12639-016-0820-3. Epub 2016 Aug 16.
A number of parasitic platyhelminthes are known to cause genotoxicity in humans and animals. However no such information is available on tropical liver fluke, , which incurs huge economic losses worldwide. In the present study the genotoxic potential of infection in rabbits, experimentally infected with the metacercarial cysts of this parasite, has been investigated using the standard comet assay and micronucleus (MNi) test on the isolated hepatocytes and the whole blood from the infected rabbits. The tail length of the comet in both hepatocytes and reticulocytes from the infected animals was significantly prominent ( < 0.05) as compared to the controls. About 61.17 % of the hepatocytes from the infected rabbits were positive for MNi formation. A number of blood cells also showed cellular deformities, which were recognised as spicule type, schistocytes, tear drop type, acanthocytes and dumbbell type. It is possible that during the establishment of host-parasite relationship the worms might have released some products which could have contributed to the induction of cellular and DNA damage. However, long term studies are required to understand the serious implications of such an effect caused by , though hepatic carcinoma has not been reported so far due to fasciolosis, however, considering the present results the possibility may not be rule out for the disease progression in this direction.
已知许多寄生扁形虫会对人类和动物造成基因毒性。然而，对于在全球范围内造成巨大经济损失的热带肝吸虫，尚无此类信息。在本研究中，通过对感染该寄生虫后尾蚴囊的家兔进行实验感染，使用标准彗星试验和微核（MNi）试验，对感染家兔的分离肝细胞和全血进行了基因毒性潜力研究。与对照组相比，感染动物的肝细胞和网织红细胞中的彗星尾长均显著突出（P < 0.05）。感染家兔约61.17%的肝细胞微核形成呈阳性。许多血细胞也表现出细胞畸形，被识别为针状、裂体细胞、泪滴状、棘状细胞和哑铃状。在宿主 - 寄生虫关系建立过程中，蠕虫可能释放了一些产物，这些产物可能导致了细胞和DNA损伤。然而，尽管目前尚未有因肝片吸虫病导致肝癌的报道，但需要长期研究来了解由热带肝吸虫造成的这种影响的严重后果，考虑到目前的结果，不能排除疾病朝这个方向发展的可能性。
