Ngo Quynh-Mai Thi, Lee Hyun-Su, Nguyen Van Thu, Kim Jeong Ah, Woo Mi Hee, Min Byung Sun
College of Pharmacy, Drug Research and Development Center, Catholic University of Daegu, Gyeongbuk, 38430, Republic of Korea.
College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
Phytochemistry. 2017 Sep;141:147-155. doi: 10.1016/j.phytochem.2017.06.001. Epub 2017 Jun 12.
A previously undescribed nor-dammarane, 3β,20,23-trihydroxy-24,25,26,27-tetranordammarane; three previously undescribed secoiridoid glycosides, ligujaponosides A-B, and iso-oleonuzhenide; and twenty three known compounds, were isolated from the fruits of Ligustrum japonicum Thunb. Their chemical structures were elucidated by extensive spectroscopic analyses, including 1D and 2D NMR, and HRMS. The isolated compounds were screened for immunosuppressive effects on T activated cells by evaluating interleukin-2 (IL-2) production. Among them, sesamin inhibited IL-2 production in Jurkat T cells with an IC value of 38 ± 2 μM. In addition, sesamin inhibited the phosphorylation of extracellular signal-regulated protein kinase (ERK), a member of the mitogen-activated protein kinase (MAPK) family, in phorbol 12-myristate 13-acetate (PMA)/A23187-stimulated T cells. Therefore, sesamin was demonstrated to inhibit T cell activation via regulation of MAPK phosphorylation pathway.
从日本女贞的果实中分离出一种此前未被描述的降达玛烷型化合物3β,20,23-三羟基-24,25,26,27-四降达玛烷；三种此前未被描述的裂环环烯醚萜苷类化合物，即女贞日本苷A - B和异橄榄贞苷；以及二十三种已知化合物。通过广泛的光谱分析，包括一维和二维核磁共振以及高分辨质谱，阐明了它们的化学结构。通过评估白细胞介素-2（IL - 2）的产生，对分离出 的化合物进行了对T激活细胞免疫抑制作用的筛选。其中，芝麻素抑制Jurkat T细胞中IL - 2的产生，IC值为38±2 μM。此外，芝麻素在佛波酯12 - 肉豆蔻酸酯13 - 乙酸酯（PMA）/A23187刺激的T细胞中抑制细胞外信号调节蛋白激酶（ERK，丝裂原活化蛋白激酶（MAPK）家族的一员）的磷酸化。因此，芝麻素被证明通过调节MAPK磷酸化途径抑制T细胞活化。
