Department of Biochemistry, Molecular Neuroscience Center, and Biotechnology Research Institute, The Hong Kong University of Science and Technology, Hong Kong SAR, China.
Planta Med. 2010 Jul;76(10):998-1003. doi: 10.1055/s-0029-1240869. Epub 2010 Feb 8.
Two new secoiridoid glucosides, namely iso-oleonuezhenide (1) and methyloleoside 7-ethyl ester (2), along with five known ones, oleonuezhenide (3), nuezhenide (4), oleuropein (5), G13 (6), and jaspolyside methyl ester (7), were isolated from the fruits of Ligustrum lucidum. Their structures were assigned based on 1H-NMR, 13C-NMR, and 2D-NMR analyses, in combination with HR-MS experiments and the comparison with literature data of related compounds, as well as on chemical experiments. We have examined the ability of these compounds to activate ERK and CREB in cultured cortical neurons. Our studies demonstrate that compound 1 induces ERK and CREB phosphorylation in primary cortical neurons in a dose- and temporal-dependent manner, suggesting its bioactivity on neurons.
从女贞果实中分离得到了两种新的环烯醚萜苷类化合物，即 iso-oleonuezhenide（1）和 methyloleoside 7-ethyl ester（2），以及 5 种已知化合物，oleonuezhenide（3）、nuezhenide（4）、橄榄苦苷（5）、G13（6）和 jaspolyside 甲酯（7）。它们的结构是根据 1H-NMR、13C-NMR 和 2D-NMR 分析，并结合 HR-MS 实验和相关化合物文献数据的比较以及化学实验来确定的。我们已经检测了这些化合物在培养的皮质神经元中激活 ERK 和 CREB 的能力。我们的研究表明，化合物 1 以剂量和时间依赖性的方式诱导原代皮质神经元中 ERK 和 CREB 的磷酸化，表明其对神经元具有生物活性。
