Gong Huangbo, Wen Hao, Zhu Xuhui, Lian Yifan, Yang Xiaojun, Qian Zhuyin, Zhu Jin
1 The Second Clinical Medical College of Nanjing Medical University, Nanjing, China.
2 Pancreas Center, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Tumour Biol. 2017 Jun;39(6):1010428317705336. doi: 10.1177/1010428317705336.
This study aims to investigate the function of long non-coding RNA ZEB1-AS1, reveal its molecular mechanism in colorectal cancer cell growth, and evaluate its clinical significance in colorectal cancer patients. ZEB1-AS1 has reported in the development of several cancers, but the biological role of it in colorectal cancer has not been discussed. In this report, ZEB1-AS1 expression level was measured with quantitative real-time polymerase chain reaction in 63 pairs of colorectal cancer tissues and paired adjacent non-tumor colorectal tissues. The relationship between ZEB1-AS1 expression and overall survival was analyzed by virtue of Kaplan-Meier analysis. Subsequently, small interfering RNA or lentivirus vector-mediated lncRNA ZEB1-AS1 was transfected into colorectal cancer cell lines. Cell viability and apoptosis were examined. Later, nude mouse transplantation experiment was conducted to evaluate the effect of ZEB1-AS1 on colorectal cancer development in vivo. It turns out that ZEB1-AS1 is upregulated in colorectal cancer tissues and its expression is significantly associated with overall survival rate and recurrence-free survival. Upregulation of ZEB1-AS1 colorectal cancer promotes cell proliferation and inhibits cell apoptosis. In addition, cell cycle inhibitory protein p15 participates in the oncogenic function of ZEB1-AS1. Collectively, ZEB1-AS1 has asignificant effect on colorectal cancer pathological process and serves as a valuable prognostic biomarker for colorectal cancer.
本研究旨在探讨长链非编码RNA ZEB1-AS1的功能，揭示其在结直肠癌细胞生长中的分子机制，并评估其在结直肠癌患者中的临床意义。ZEB1-AS1已在多种癌症的发生发展中被报道，但其在结直肠癌中的生物学作用尚未见讨论。在本报告中，采用定量实时聚合酶链反应检测了63对结直肠癌组织及配对的相邻非肿瘤结直肠组织中ZEB1-AS1的表达水平。借助Kaplan-Meier分析方法分析了ZEB1-AS1表达与总生存期的关系。随后，将小干扰RNA或慢病毒载体介导的lncRNA ZEB1-AS1转染至结直肠癌细胞系。检测细胞活力和凋亡情况。之后，进行裸鼠移植实验以评估ZEB1-AS1对体内结直肠癌发展的影响。结果显示，ZEB1-AS1在结直肠癌组织中表达上调，其表达与总生存率和无复发生存率显著相关。ZEB1-AS1的上调促进结直肠癌细胞增殖并抑制细胞凋亡。此外，细胞周期抑制蛋白p15参与了ZEB1-AS1的致癌功能。综上所述，ZEB1-AS1对结直肠癌病理过程具有显著影响，可作为结直肠癌有价值的预后生物标志物。
