Liu Hao, Sun Xiaowei, Zou Wei, Leng Mengtong, Zhang Beng, Kang Xiaoyu, He Tao, Wang Hui
Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang Province, China.
First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang Province, China.
Complement Ther Med. 2017 Jun;32:85-90. doi: 10.1016/j.ctim.2017.03.014. Epub 2017 Apr 19.
Hemorrhagic stroke accounts for approximately 15% of all stroke cases, and is associated with high morbidity and mortality. Limited human studies suggested that scalp acupuncture could facilitate functional recovery after cerebral hemorrhage. In the current study, we used an animal model of cerebral hemorrhage to examine the potential effects of scalp acupuncture.
Adult male Sprague-Dawley rats received autologous blood (50μL) into the right caudate nucleus on the right side under pentobarbital anesthesia, and then received scalp acupuncture (DU20 through GB7 on the lesion side) or sham acupuncture (1cm to the right side of the acupoints) (n=10 per group). A group of rats receiving autologous blood into the caudate nucleus but no other intervention, as well as a group of rats receiving anesthesia but no blood injection to the brain (n=10 per group) were included as additional controls. Composite neuroscore, corner turn test, forelimb placing test, wire hang task and beam walking were used to evaluate the behavior of rats. Hematoxylin and Eosin (HE) staining was used to observe the histopathological changes. Western blot was used to detect the content of tumor necrosis factor alpha (TNF-α) and nuclear factor-KappaB (NFκB) protein expression.
Scalp acupuncture attenuated neurological deficits (p<0.01 or <0.05 vs. sham acupuncture using a variety of behavioral tests) at 1-7days after the treatment. The brain content of TNF-α and NFκB was decreased (p<0.01 for both).
Scalp acupuncture could improve neurological deficits in a rat model of hemorrhagic stroke.
出血性中风约占所有中风病例的15%，且与高发病率和高死亡率相关。有限的人体研究表明，头皮针疗法可促进脑出血后的功能恢复。在本研究中，我们使用脑出血动物模型来研究头皮针疗法的潜在作用。
成年雄性Sprague-Dawley大鼠在戊巴比妥麻醉下于右侧尾状核注入自体血（50μL），然后接受头皮针治疗（病变侧从督脉20穴至足少阳胆经7穴）或假针刺（穴位右侧1cm处）（每组n = 10）。将一组仅在尾状核注入自体血但未接受其他干预的大鼠，以及一组仅接受麻醉但未向脑内注射血液的大鼠（每组n = 10）作为额外对照。采用综合神经评分、转角试验、前肢放置试验、悬线任务和横梁行走试验来评估大鼠的行为。采用苏木精-伊红（HE）染色观察组织病理学变化。采用蛋白质免疫印迹法检测肿瘤坏死因子α（TNF-α）含量及核因子κB（NFκB）蛋白表达。
治疗后1 - 7天，头皮针疗法减轻了神经功能缺损（与假针刺相比，多种行为测试中p < 0.01或< 0.05）。TNF-α和NFκB的脑内含量降低（两者均p < 0.01）。
头皮针疗法可改善出血性中风大鼠模型的神经功能缺损。
