Eddy F B, Talbot C
Comp Biochem Physiol C Comp Pharmacol Toxicol. 1985;81(2):259-66. doi: 10.1016/0742-8413(85)90003-9.
Ionic regulation of eyed eggs of Atlantic salmon was studied using intact eggs and embryos from which the chorion and perivitelline fluid had been removed, a preparation which remained in good condition for at least 2 weeks. Both intact eggs and embryos in freshwater showed similar Na+ influx rates (0.0064 and 0.01 microM g-1 hr-1, respectively) but Na+ efflux rates (0.0024 and 0.0064 microM g-1 hr-1, respectively) were greater in embryos, suggesting that the perivitelline fluid which is known to bind cations has an important function in preventing Na+ loss from the yolk and embryo. Over 90% of the egg Na+ is located in the yolk and embryo but only about 10% is exchangeable while the chorion contains about 8% Na+ which is non-exchangeable. Both eggs and embryos in acid water at pH 4, in Zn2+ 5 mM l-1 and in aluminium 1 mM l-1 showed greatly reduced Na+ uptake but eggs in 10 microM l-1 aluminium or 100 microM l-1 Zn2+ showed normal Na+ balance while embryos were normal in 10 microM l-1 aluminium but showed reduced Na+ uptake in 100 microM l-1 Zn2+. It is concluded that the chorion and perivitelline fluid have a capacity to protect the embryo from metal ions and acid water.
利用完整的鱼卵和去除了卵膜及卵周液的胚胎，对大西洋鲑有眼卵的离子调节进行了研究，这种制备物在至少两周内保持良好状态。淡水环境中的完整鱼卵和胚胎显示出相似的Na⁺流入速率（分别为0.0064和0.01微摩尔/克·小时），但胚胎中的Na⁺流出速率（分别为0.0024和0.0064微摩尔/克·小时）更高，这表明已知能结合阳离子的卵周液在防止Na⁺从卵黄和胚胎中流失方面具有重要作用。超过90%的鱼卵Na⁺位于卵黄和胚胎中，但只有约10%是可交换的，而卵膜含有约8%不可交换的Na⁺。pH值为4的酸性水中、5毫摩尔/升的Zn²⁺以及1毫摩尔/升的铝中的鱼卵和胚胎均显示出Na⁺摄取大幅减少，但10微摩尔/升铝或100微摩尔/升Zn²⁺中的鱼卵显示出正常的Na⁺平衡，而10微摩尔/升铝中的胚胎正常，但在100微摩尔/升Zn²⁺中显示出Na⁺摄取减少。研究得出结论，卵膜和卵周液有能力保护胚胎免受金属离子和酸性水的影响。
